Change search
ReferencesLink to record
Permanent link

Direct link
Development and evaluation of a broad reacting SYBR-green based quantitative real-time PCR for the detection of different hantaviruses
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
Show others and affiliations
2013 (English)In: Journal of Clinical Virology, ISSN 1386-6532, E-ISSN 1873-5967, Vol. 56, no 4, 280-285 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Hantaviruses are endemic in most parts of the world and cause hundreds of thousand human cases of hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS) annually throughout Eurasia and the Americas. They are zoonotic viruses, most commonly transmitted to humans by aerosolized rodent excreta. New hantaviruses are frequently discovered in previously unknown reservoir species and geographic areas. Consequently, there is a need to improve hantavirus diagnostics. OBJECTIVES: This paper describes the design and evaluation of a rapid and robust quantitative real-time PCR (QRT-PCR) assay able to detect a wide range of hantaviruses. STUDY DESIGN: Primers with the potential to detect different hantaviruses were designed from conserved regions of different hantavirus L segments, as identified from multiple sequence alignments. RESULTS: By using SYBR-green-based QRT-PCR 100-1000 target molecules of in vitro produced RNA and less than 100 copies of hantavirus RNA from different hantavirus clades and regions of the world were detected. When using the assay on clinical samples from patients with acute HFRS, Puumala hantavirus (PUUV) RNA was confirmed in all previously positive samples. Notably, the broad reacting L-segment QRT-PCR also detected viral RNA in HFRS patient samples, previously negative by a QRT-PCR targeting the S segment of PUUV. CONCLUSIONS: This novel assay provides a powerful tool for diagnosis of hantaviruses from different clades and regions and may also be useful in surveys with the purpose of finding new hantaviruses in rodent or insectivore species.

Place, publisher, year, edition, pages
2013. Vol. 56, no 4, 280-285 p.
National Category
Microbiology in the medical area
URN: urn:nbn:se:umu:diva-64511DOI: 10.1016/j.jcv.2012.12.001ISI: 000316657400002PubMedID: 23290388OAI: diva2:602074
Available from: 2013-01-31 Created: 2013-01-31 Last updated: 2016-05-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Mohamed, NahlaEvander, MagnusAhlm, Clas
By organisation
Infectious DiseasesVirology
In the same journal
Journal of Clinical Virology
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 85 hits
ReferencesLink to record
Permanent link

Direct link