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YopH of Yersinia pseudotuberculosis interrupts early phosphotyrosine signalling associated with phagocytosis.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Lundgren)
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1996 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 20, no 5, 1057-69 p.Article in journal (Refereed) Published
Abstract [en]

The PTPase YopH of Yersinia is essential to the ability of these bacteria to block phagocytosis. Wild-type Yersinia pseudotuberculosis, but not the yopH mutant strain, resisted phagocytosis by J774 cells. Ingestion of a yopH mutant was dependent on tyrosine kinase activity. Transcomplementation with wild-type yopH restored the anti-phagocytic effect, whereas introduction of the gene encoding the catalytically inactive yopHC403A was without effect. The PTPase inhibitor orthovanadate impaired the anti-phagocytic effect of the wild-type strain, further demonstrating the importance of bacteria-derived PTPase activity for this event. The ability to resist phagocytosis indicates that the effect of the bacterium is immediately exerted when it becomes associated with the phagocyte. Within 30 s after the onset of infection, wild-type Y. pseudotuberculosis caused a YopH-dependent dephosphorylation of phosphotyrosine proteins in J774 cells. Furthermore, interaction of the cells with phagocytosable strains led to a rapid and transient increase in tyrosine phosphorylation of paxillin and some other proteins, an event dependent on the presence of the bacterial surface-located protein invasin. Co-infection with the phagocytosable strain and the wild-type strain abolished the induction of tyrosine phosphorylation. Taken together, the present findings demonstrate an immediate YopH-mediated dephosphorylation of macrophage phosphotyrosine proteins, suggesting that this PTPase acts by preventing early phagocytosis-linked signalling in the phagocyte.

Place, publisher, year, edition, pages
1996. Vol. 20, no 5, 1057-69 p.
URN: urn:nbn:se:umu:diva-65076PubMedID: 8809758OAI: diva2:603220
Available from: 2013-02-05 Created: 2013-02-05 Last updated: 2013-02-06
In thesis
1. Interactions between Yersinia pseudotuberculosis and host cells - role of invasin and YopH
Open this publication in new window or tab >>Interactions between Yersinia pseudotuberculosis and host cells - role of invasin and YopH
1999 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Umeå: Umeå universitet, 1999. 72 p.
Umeå University medical dissertations, ISSN 0346-6612 ; 634
National Category
Medical and Health Sciences
urn:nbn:se:umu:diva-65079 (URN)91-7191-732-2 (ISBN)
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Föreläsningssalen, Institutionen för mikrobiologi, Umeå universitet, Umeå (English)

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Available from: 2013-02-06 Created: 2013-02-05 Last updated: 2013-02-06Bibliographically approved

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Wolf-Watz, HFällman, M
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