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Modulation of α-synuclein fibrillization by ring-fused 2-pyridones: Templation and inhibition involve oligomers with different structure
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2013 (English)In: Archives of Biochemistry and Biophysics, ISSN 0003-9861, E-ISSN 1096-0384, Vol. 532, no 2, 84-90 p.Article in journal (Refereed) Published
Abstract [en]

In a recent study we discovered that a ring-fused 2-pyridone compound triggered fibrillization of a key protein in Parkinson's disease, α-synuclein. To reveal how variations in compound structure affect protein aggregation, we now prepared a number of strategic analogs and tested their effects on α-synuclein amyloid fiber formation in vitro. We find that, in contrast to the earlier templating effect, some analogs inhibit α-synuclein fibrillization. For both templating and inhibiting compounds, the key species formed in the reactions are α-synuclein oligomers that contain compound. Despite similar macroscopic appearance, the templating and inhibiting oligomers are distinctly different in secondary structure content. When the inhibitory oligomers are added in seed amounts, they inhibit fresh α-synuclein aggregation reactions. Our study demonstrates that small chemical changes to the same central fragment can result in opposite effects on protein aggregation.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 532, no 2, 84-90 p.
Keyword [en]
α-Synuclein, Amyloid, Oligomer, Protein aggregation, Spectroscopy, 2-Pyridone
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-66116DOI: 10.1016/j.abb.2013.01.012PubMedID: 23399432OAI: oai:DiVA.org:umu-66116DiVA: diva2:605629
Available from: 2013-02-14 Created: 2013-02-14 Last updated: 2017-12-06Bibliographically approved

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Horvath, IstvanSellstedt, MagnusWeise, ChristophNordvall, Lina-MariaGolla, Krishna PrasadOlofsson, AndersLarsson, GöranAlmqvist, FredrikWittung-Stafshede, Pernilla
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Department of ChemistryDepartment of Medical Biochemistry and Biophysics
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