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Functional multiplex reporter assay using tagged Gaussia luciferase
Umeå University, Faculty of Medicine, Department of Radiation Sciences, Oncology.
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2013 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 3, article id 1046Article in journal (Refereed) Published
Abstract [en]

We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc tag), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc tag detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.

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Nature Publishing Group, 2013. Vol. 3, article id 1046
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Cancer and Oncology
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URN: urn:nbn:se:umu:diva-67304DOI: 10.1038/srep01046ISI: 000313418100001PubMedID: 23308339OAI: oai:DiVA.org:umu-67304DiVA, id: diva2:611993
Available from: 2013-03-19 Created: 2013-03-15 Last updated: 2018-01-11Bibliographically approved

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  • de-DE
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