Functional multiplex reporter assay using tagged Gaussia luciferase
2013 (English)In: Scientific Reports, ISSN 2045-2322, Vol. 3, 1046- p.Article in journal (Refereed) Published
We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc tag), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc tag detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.
Place, publisher, year, edition, pages
2013. Vol. 3, 1046- p.
Cancer and Oncology
IdentifiersURN: urn:nbn:se:umu:diva-67304DOI: 10.1038/srep01046OAI: oai:DiVA.org:umu-67304DiVA: diva2:611993
Art. No.: 10462013-03-192013-03-152013-03-19Bibliographically approved