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Functional multiplex reporter assay using tagged Gaussia luciferase
Umeå University, Faculty of Medicine, Department of Radiation Sciences, Oncology.
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2013 (English)In: Scientific Reports, ISSN 2045-2322, Vol. 3, 1046- p.Article in journal (Refereed) Published
Abstract [en]

We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc tag), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc tag detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.

Place, publisher, year, edition, pages
2013. Vol. 3, 1046- p.
National Category
Cancer and Oncology
URN: urn:nbn:se:umu:diva-67304DOI: 10.1038/srep01046OAI: diva2:611993

Art. No.: 1046

Available from: 2013-03-19 Created: 2013-03-15 Last updated: 2013-03-19Bibliographically approved

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