Change search
ReferencesLink to record
Permanent link

Direct link
Cytokine profiles for human V gamma 9+ T cells stimulated by Plasmodium falciparum.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Immunology/Immunchemistry.
Show others and affiliations
1995 (English)In: Parasite immunology (Print), ISSN 0141-9838, E-ISSN 1365-3024, Vol. 17, no 8, 413-23 p.Article in journal (Refereed) Published
Abstract [en]

V gamma 9+ T cells from malaria non-exposed donors make proliferative responses to Plasmodium falciparum on in vitro stimulation. V gamma 9+ cells are strongly activated by components of the schizont stage of the parasite and by antigens released into the culture upon schizogony, while CD4+V gamma 9- cells are stimulated by the earlier stages of the parasite. Using reverse transcriptase-polymerase chain reaction (RT-PCR) we determined mRNA expression for 14 cytokines in highly purified V gamma 9+ cells enriched by positive selection after in vitro stimulation with P. falciparum schizont antigens. Interferon-gamma (IFN-gamma) and Tumor Necrosis Factor-alpha (TNF-alpha) were detected in all samples tested. The majority of samples also expressed TNF-beta, transforming growth factor-beta (TGF-beta) and Interleukin-8 (IL-8). Only occasional samples expressed IL-2, IL-5 and IL-10. Using the ELISPOT assay we found that a large fraction of the reactive V gamma 9+ cells produced IFN-gamma and that gamma delta T cells are the major producers of IFN-gamma in cultures stimulated with schizont antigens. The majority of V gamma 9+ cells in these cultures also express the membrane-bound form of TNF-alpha. Expression of these cytokines speaks for a cytolytic and/or inflammatory role of gamma delta cells in the response to malaria in non-exposed individuals.

Place, publisher, year, edition, pages
1995. Vol. 17, no 8, 413-23 p.
National Category
URN: urn:nbn:se:umu:diva-67899PubMedID: 7501422OAI: diva2:614792
Available from: 2013-04-07 Created: 2013-04-07 Last updated: 2013-04-07

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Hammarström, Marie-Louise
By organisation
In the same journal
Parasite immunology (Print)

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 13 hits
ReferencesLink to record
Permanent link

Direct link