Water uptake on polar stationary phases under conditions for hydrophilic interaction chromatography and its relation to solute retention
2013 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1320, 33-47 p.Article in journal (Other academic) Published
In hydrophilic interaction chromatography, water is known to accumulate on the stationary phase to form a water enriched layer, which is believed to play an important role in the retention mechanism. To gain a better understanding retention mechanism in HILIC, we have determined the water uptake on twelve different HILIC stationary phases. Non-modified and monomerically functionalized silica phases followed a pattern of monolayer formation followed by multiple layer adsorption, while the water uptake on polymerically functionalized silica stationary phase showed the characteristics of formation and swelling of hydrogels. This difference in the nature of water accumulation was found to be related to different water uptake patterns when methanol and tetrahydrofuran were added to 80:20 % (v/v) acetonitrile/water by replacing 5 % of the acetonitrile as tertiary solvents, and also when ammonium acetate was added as buffering electrolyte. The relationship between water uptake and retention mechanism was investigated by looking at the correlation between retention factors of neutral analytes and phase ratios of HILIC columns, calculated either as surface area (adsorption) or volume of the water layer enriched from the acetonitrile/water eluent (partitioning). Regardless of the adsorption or partitioning mechanism, the interaction of neutral analytes and stationary phase could be mainly the hydrogen bonding between analytes and the accumulated water in the water enriched layer.
Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 1320, 33-47 p.
HILIC, HPLC, Retention mechanism, Stationary phases, Eluents
Analytical Chemistry Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-68067DOI: 10.1016/j.chroma.2013.09.061ISI: 000327922100005OAI: oai:DiVA.org:umu-68067DiVA: diva2:615721
FunderSwedish Research Council, 2008-3621, 2012-4000
Manuscript version included in dissertation with the title: Water uptake on polar stationary phases under HILIC conditions and its relation to solute retention2013-04-112013-04-112014-02-25Bibliographically approved