Investigations of the retention mechanisms in hydrophilic interaction chromatography
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Hydrophilic interaction chromatography is well known as a powerful technique separation of polar and ionizable compound nowadays. However the retention mechanism of the technique is still under debate. Understanding retention mechanism would facilitate the method development using the technique and its future improvement. This was inspiring and became the goal of this thesis.
This work involves the characterization of the water enriched layer regarding to water and buffer salt accumulation. Twelve HILIC stationary phase with a diverse surface chemistry regarding to function groups and modification type were studied. Effect of water and salt on regarding to the retention mechanism was investigated by correlating the adsorption data to the retention of selected solutes
This also involved the characterization of interactions involve in the separation of 21 HILIC columns. Interactions was probe by retention ratio of pair solutes which are characteristic for each specific interaction. The data was evaluate using principle component analysis – a multivariable data analysis method. The model was comprehensive and its outcomes were confirmed by the studies on adsorptions of water and salts.
Place, publisher, year, edition, pages
Umeå: Umeå University , 2013. , 42 p.
HILIC, hydrophilic interaction chromatography, adsorption isotherm, water enriched layer, multivariate data analysis, retention mechanism, separation
Research subject Analytical Chemistry
IdentifiersURN: urn:nbn:se:umu:diva-68071ISBN: 978-91-7459-630-4OAI: oai:DiVA.org:umu-68071DiVA: diva2:615745
2013-05-03, KBC Building, Room KB3A3, Umeå universitet, Umeå, 10:00 (English)
Alpert, Andrew J., Dr
Irgum, Knut, Professor
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