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The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric contractions.
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Sports Medicine.
Department of Cell Biology, Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
2003 (English)In: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119X, Vol. 119, no 5, 383-93 p.Article in journal (Refereed) Published
Abstract [en]

Myofibrillar Z-disc streaming and loss of the desmin cytoskeleton are considered the morphological hallmarks of eccentric contraction-induced injury. The latter is contradicted by recent studies where a focal increase of desmin was observed in biopsies taken from human muscles with DOMS. In order to determine the effects of eccentric contraction-induced alterations of the myofibrillar Z-disc, we examined the distribution of alpha-actinin, the Z-disc portion of titin and the nebulin NB2 region in relation to actin and desmin in DOMS biopsies. In biopsies taken 2-3 days and 7-8 days after exercise, we observed a significantly higher number of fibres showing focal areas lacking staining for alpha-actinin, titin and nebulin than in biopsies taken from control or 1 h after exercise. None of these proteins were part of Z-disc streamings but instead they were found in distinct patterns in areas characterised by altered staining for desmin and actin. These were preferentially seen in regions with increased numbers of sarcomeres in parallel myofibrils. We propose that these staining patterns represent different stages of sarcomere formation. These findings therefore support our previous suggestion that muscle fibres subjected to eccentric contractions adapt to unaccustomed activity by the addition of new sarcomeres.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2003. Vol. 119, no 5, 383-93 p.
National Category
Sport and Fitness Sciences
URN: urn:nbn:se:umu:diva-68831DOI: 10.1007/s00418-003-0522-7PubMedID: 12712356OAI: diva2:618043
Available from: 2013-04-25 Created: 2013-04-25 Last updated: 2013-06-03Bibliographically approved

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Yu, Ji-GuoThornell, Lars-Eric
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