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Broad feedback inhibition of pre-B-cell receptor signaling components
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
2013 (English)In: Molecular Immunology, ISSN 0161-5890, E-ISSN 1872-9142, Vol. 54, no 3-4, 247-253 p.Article in journal (Refereed) Published
Abstract [en]

During B lymphocyte development, first immunoglobulin heavy chain gene segments and then immunoglobulin light chain gene segments are rearranged to create antibody diversity. Early in the development, expression of a pre-B-cell receptor (pre-BCR) that has membrane-bound Ig heavy chain protein associated with surrogate light chain (SLC) proteins serves as a critical checkpoint that monitors for functional heavy chain rearrangement. Signaling from the pre-BCR induces survival and clonal expansion to select cells with good heavy chains, but it also down-regulates transcription of the genes for the SLC proteins and CD19 and limits its own proliferative signaling. Here we have analyzed whether the down-regulation is limited to the SLC proteins and CD19, and we show that the pre-BCR of primary mouse pre-B-cells instead is subject to a broad feedback inhibition of pre-BCR signaling components. Activation of signaling leads to down-regulation of the receptor proteins, many co-receptors and proteins participating in signal pathways from the receptor. Thus the down-regulation of the pre-BCR is much broader than previously assumed. We also show that Ca2+/calmodulin inhibition of the transcription factor E2A is required for the feedback inhibition of the pre-BCR signaling proteins. (C) 2012 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 54, no 3-4, 247-253 p.
Keyword [en]
Pre-BCR, Calcium signaling, Cell proliferation, Gene regulation, E2A, Calmodulin, Signal transduction
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-70330DOI: 10.1016/j.molimm.2012.12.002ISI: 000317157000001OAI: oai:DiVA.org:umu-70330DiVA: diva2:621328
Available from: 2013-05-14 Created: 2013-05-14 Last updated: 2017-12-06Bibliographically approved

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Hauser, JannekVerma-Gaur, JiyotiGrundström, Thomas
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CiteExportLink to record
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