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Ultrasensitive optical detection of hydrogen peroxide by triggered activation of horseradish peroxidase
CIC biomaGUNE, Parque Tecnologico de San Sebastian, Paseo Miramon 182, San Sebastian, Spain .
2010 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 135, no 9, 2291-2295 p.Article in journal (Refereed) Published
Abstract [en]

Hydrogen peroxide is a very reactive byproduct of many metabolic pathways. We describe an ultra-sensitive colorimetric method to detect hydrogen peroxide based on the reconstitution of apo-horseradish peroxidase with the hemin derivative, hemin di(N,N'-acetyl-hydrazide). Oxidation of the latter by hydrogen peroxide yields hemin, which is able to reconstitute apo-horseradish peroxidase, forming an active peroxidase. We have also applied this analyte-triggered reconstitution of horseradish peroxidase to detect the activity of the enzyme glucose oxidase.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2010. Vol. 135, no 9, 2291-2295 p.
Keyword [en]
amino acid phenylhydrazides, colorimetric assay, peptide synthesis, destructive oxidation, haemin, reconstitution, amplification, derivatives, catalysts, oxidase
National Category
Analytical Chemistry Basic Medicine
Identifiers
URN: urn:nbn:se:umu:diva-73233DOI: 10.1039/c0an00095gISI: 000281007300013PubMedID: 20464020OAI: oai:DiVA.org:umu-73233DiVA: diva2:630462
Available from: 2013-06-19 Created: 2013-06-19 Last updated: 2017-12-06Bibliographically approved

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