Ultrasensitive optical detection of hydrogen peroxide by triggered activation of horseradish peroxidase
2010 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 135, no 9, 2291-2295 p.Article in journal (Refereed) Published
Hydrogen peroxide is a very reactive byproduct of many metabolic pathways. We describe an ultra-sensitive colorimetric method to detect hydrogen peroxide based on the reconstitution of apo-horseradish peroxidase with the hemin derivative, hemin di(N,N'-acetyl-hydrazide). Oxidation of the latter by hydrogen peroxide yields hemin, which is able to reconstitute apo-horseradish peroxidase, forming an active peroxidase. We have also applied this analyte-triggered reconstitution of horseradish peroxidase to detect the activity of the enzyme glucose oxidase.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2010. Vol. 135, no 9, 2291-2295 p.
amino acid phenylhydrazides, colorimetric assay, peptide synthesis, destructive oxidation, haemin, reconstitution, amplification, derivatives, catalysts, oxidase
Analytical Chemistry Basic Medicine
IdentifiersURN: urn:nbn:se:umu:diva-73233DOI: 10.1039/c0an00095gISI: 000281007300013PubMedID: 20464020OAI: oai:DiVA.org:umu-73233DiVA: diva2:630462