umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The use of fluorescence techniques for the study of some membrane-bound photosynthetic properties and some effects of copper on the thylakoid membrane
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.ORCID iD: 0000-0003-2133-6526
1981 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Lyophilized pea chloroplasts were extracted in a stepwise manner in an organic solvent (petroleum ether) with increasing polarity which was obtained by addition of small amount of ETOH (0-1 %). Absorption and low temperature fluorescence emission spectra were measured on both the extracted thylakoids and on isolated chlorophylI-protein complexes. Extraction of chlorophyll from the membrane increased (and the ratio of chlorophyl l a/b decreased) with increasing polarity of the solvent. The gel scan revealed that after extraction with petroleum ether, CPa.. was lost from the gel and after extraction with petroleum ether +1 % ETOH only the CPa/b was left together with SDS-free chlorophyll. This shows that the chlorophyll in CPa/b are situated in a less hydrophobic environment than chlorophyll in CPa|| and CPa|. The long wavelength absorbing and emitting chlorophyll fraction associated to CPaj was found to be easily removed from the membrane. This caused a blue shift in the low temperature fluorescence emission peak and in the red absorption peak and it was also accompanied by a decrease in carote-noid absorption in isolated CPa|. It was found in different plant material lacking $-carotene in CPàj that a strong correlation between ß-carotene in CPa. and the existence of the long wavelength chlorophyll in isolated cPa. existed. Based on these data, it was suggested that excited chlorophyll can transfer energy in excess to ß-carotene by a triplet--triplet transfer.A method based on in vivo chlorophyll £ fluorescence was developed for studying photosynthetic capacity in unicellular algae. It was shown that DCMU-induced fluorescence increase was a good measure of photosynthetic capacity in four species of green algae tested.The effect of copper chloride on photosynthetic electron transport and chlorophyl1-protein complexes was studied in spinach chloroplasts. Copper(11) inhibited a PS I i reaction H2O—> DPIP, a PS I reaction Asc/DPIP —> NADP and the overall electron transport H2O —> NAOP to different degrees. Chlorophyll protein complexes were only slightly affected by copper(ll) but with both copper and ascorbate in the reaction media, a rapid membrane destruction occurred. This was probably caused by a free radical reaction catalyzed by copper(ll).

Place, publisher, year, edition, pages
Umeå: Umeå universitet , 1981. , 19 p.
Keyword [en]
chlorophylI-protein complexes, sequential extraction, ß-carotene low temperature fluorescence emission, absorption, in vivo chi. a fluorescence, copper, electron transport
National Category
Botany
Identifiers
URN: urn:nbn:se:umu:diva-73599ISBN: 91-7174-076-7 (print)OAI: oai:DiVA.org:umu-73599DiVA: diva2:632628
Public defence
1981-05-15, Fysologi-Botanik Hufo, seminarierum B, Umeå universitet, Umeå, 10:00
Opponent
Projects
digitalisering@umu
Note

Härtill 4 delarbeten.

Available from: 2013-06-25 Created: 2013-06-25 Last updated: 2015-04-29Bibliographically approved

Open Access in DiVA

The use of fluorescence techniques for the study of some membrane-bound photosynthetic properties and some effects of copper on the thylakoid membrane(1428 kB)180 downloads
File information
File name FULLTEXT02.pdfFile size 1428 kBChecksum SHA-512
26fb27d6718ac95a2ad30381b10cc7c9354ac633a0970eed2a4fe558856a152979559e9a527d01fa9ceb7ce6ad94cf9703c85141d6abecc6314c3b22c79fb7d0
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Samuelsson, Göran
By organisation
Department of Plant Physiology
Botany

Search outside of DiVA

GoogleGoogle Scholar
Total: 180 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 107 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf