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A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
2013 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 13, 96- p.Article in journal (Refereed) Published
Abstract [en]

Background: Many Gram-negative bacteria rely on a type VI secretion system (T6SS) to infect eukaryotic cells or to compete against other microbes. Common to these systems is the presence of two conserved proteins, in Vibrio cholerae denoted VipA and VipB, which have been shown to interact in many clinically relevant pathogens. In this study, mutagenesis of a defined region within the VipA protein was used to identify residues important for VipB binding in V. cholerae O1 strain A1552. Results: A dramatically diminished interaction was shown to correlate with a decrease in VipB stability and a loss of hemolysin co-regulated protein (Hcp) secretion and rendered the bacterium unable to compete with Escherichia coli in a competition assay. Conclusions: This confirms the biological relevance of the VipA-VipB interaction, which is essential for the T6SS activity of many important human pathogens.

Place, publisher, year, edition, pages
London, England: BioMed Central, 2013. Vol. 13, 96- p.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-73578DOI: 10.1186/1471-2180-13-96ISI: 000319046300001PubMedID: 23642157OAI: oai:DiVA.org:umu-73578DiVA: diva2:632843
Available from: 2013-06-25 Created: 2013-06-25 Last updated: 2017-12-06Bibliographically approved

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Bröms, Jeanette E.Ishikawa, TakahikoWai, Sun N.Sjöstedt, Anders

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Bröms, Jeanette E.Ishikawa, TakahikoWai, Sun N.Sjöstedt, Anders
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Microbiology in the medical area

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