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Effects of long term supplementation of anabolic androgen steroids on skeletal muscle structural and non-structural proteins
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Sports Medicine.
2013 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Testosterone and other anabolic androgen steroids (AAS) are widely used by both professional and amateur athletes, aiming at enhancing muscle strength, thus improved exercise performance. However, to our knowledge very few studies have been performed to investigate the long term effects of AAS on muscle protein metabolism. This study will evaluate the cellular expression and localization of four proteins after long term (5 to 15 years) supplementation of AAS, in muscle biopsies from strength athletes. HSPB1 and PGM-1 were selected for further study since they were registered in proteomics as proteins with significant different abundance between doped and clean athletes. In addition, two well-known muscle structural proteins, desmin and F-actin were also evaluated together with the two novel proteins, using immunohistochemistry (IHC) method. No obvious changes in distribution or location of either HSPB1 or PGM-1 were observed in longitudinal sections from doped biopsies, indicating that both proteins were not affected by AAS doping. Considering that PGM-1 is a metabolic related protein in muscle cells, long term AAS doping may not induce metabolic changes focally in muscle fibers. HSPB1 were not affected in areas with irregular staining for the two structural proteins, indicating that HSPB1 was not obviously structural relevant, but more prone to be a metabolism protein. AAS doping did induce focal changes in desmin and F-actin, may suggesting muscular remodeling at Z-disc level.

Place, publisher, year, edition, pages
2013. , 16 p.
Keyword [en]
AAS administration, long term, muscle protein metabolism, structural/non-structural proteins.
National Category
Sport and Fitness Sciences
URN: urn:nbn:se:umu:diva-78419OAI: diva2:637703
Educational program
Sports Science Programme, Sports Medicine and Coaching
Available from: 2013-08-12 Created: 2013-07-21 Last updated: 2013-08-12Bibliographically approved

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