Change search
ReferencesLink to record
Permanent link

Direct link
Dynamic gradients of an intermediate filament-like cytoskeleton are recruited by a polarity landmark during apical growth
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0003-3492-3287
Show others and affiliations
2013 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 110, no 21, E1889-E1897 p.Article in journal (Refereed) Published
Abstract [en]

Intermediate filament (IF)-like cytoskeleton emerges as a versatile tool for cellular organization in all kingdoms of life, underscoring the importance of mechanistically understanding its diverse manifestations. We showed previously that, in Streptomyces (a bacterium with a mycelial lifestyle similar to that of filamentous fungi, including extreme cell and growth polarity), the IF protein FilP confers rigidity to the hyphae by an unknown mechanism. Here, we provide a possible explanation for the IF-like function of FilP by demonstrating its ability to self-assemble into a cis-interconnected regular network in vitro and its localization into structures consistent with a cytoskeletal network in vivo. Furthermore, we reveal that a spatially restricted interaction between FilP and DivIVA, the main component of the Streptomyces polarisome complex, leads to formation of apical gradients of FilP in hyphae undergoing active tip extension. We propose that the coupling between the mechanism driving polar growth and the assembly of an IF cytoskeleton provides each new hypha with an additional stress-bearing structure at its tip, where the nascent cell wall is inevitably more flexible and compliant while it is being assembled and matured. Our data suggest that recruitment of cytoskeleton around a cell polarity landmark is a broadly conserved strategy in tip-growing cells.

Place, publisher, year, edition, pages
2013. Vol. 110, no 21, E1889-E1897 p.
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-79064DOI: 10.1073/pnas.1305358110ISI: 000320328700004OAI: diva2:640094
Available from: 2013-08-12 Created: 2013-08-06 Last updated: 2015-12-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Sandblad, Linda
By organisation
Department of Molecular Biology (Faculty of Medicine)
In the same journal
Proceedings of the National Academy of Sciences of the United States of America
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 46 hits
ReferencesLink to record
Permanent link

Direct link