Change search
ReferencesLink to record
Permanent link

Direct link
Replicative DNA polymerases
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
2013 (English)In: Cold Spring Harbor Perspectives in Biology, ISSN 1943-0264, Vol. 5, no 6, a012799- p.Article in journal (Refereed) Published
Abstract [en]

In 1959, Arthur Kornberg was awarded the Nobel Prize for his work on the principles by which DNA is duplicated by DNA polymerases. Since then, it has been confirmed in all branches of life that replicative DNA polymerases require a single-stranded template to build a complementary strand, but they cannot start a new DNA strand de novo. Thus, they also depend on a primase, which generally assembles a short RNA primer to provide a 3'-OH that can be extended by the replicative DNA polymerase. The general principles that (1) a helicase unwinds the double-stranded DNA, (2) single-stranded DNA-binding proteins stabilize the single-stranded DNA, (3) a primase builds a short RNA primer, and (4) a clamp loader loads a clamp to (5) facilitate the loading and processivity of the replicative polymerase, are well conserved among all species. Replication of the genome is remarkably robust and is performed with high fidelity even in extreme environments. Work over the last decade or so has confirmed (6) that a common two-metal ion-promoted mechanism exists for the nucleotidyltransferase reaction that builds DNA strands, and (7) that the replicative DNA polymerases always act as a key component of larger multiprotein assemblies, termed replisomes. Furthermore (8), the integrity of replisomes is maintained by multiple protein-protein and protein-DNA interactions, many of which are inherently weak. This enables large conformational changes to occur without dissociation of replisome components, and also means that in general replisomes cannot be isolated intact.

Place, publisher, year, edition, pages
2013. Vol. 5, no 6, a012799- p.
National Category
Medical Genetics
URN: urn:nbn:se:umu:diva-79274DOI: 10.1101/cshperspect.a012799ISI: 000320768600001OAI: diva2:645114
Available from: 2013-09-03 Created: 2013-08-13 Last updated: 2013-09-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Johansson, Erik
By organisation
Department of Medical Biochemistry and Biophysics
In the same journal
Cold Spring Harbor Perspectives in Biology
Medical Genetics

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 62 hits
ReferencesLink to record
Permanent link

Direct link