umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Age-related impaired affinity maturation and differential D-JH gene usage in human VH6-expressing B lymphocytes from healthy individuals
Division for Clinical Immunology, Karolinska Institute, Huddinge Hospital, Sweden..
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
ivision for Clinical Immunology, Karolinska Institute, Huddinge Hospital, Sweden..
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Show others and affiliations
1997 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 27, no 6, 1381-1386 p.Article in journal (Refereed) Published
Abstract [en]

To elucidate the basic molecular events underlying humoral immunity during ontogeny and senescence, we analyzed a panel of 179 polymerase chain reaction-derived VH6-D-JH rearrangements from cord blood, peripheral blood, and spleen. Nucleotide sequence analysis of the CDR3 region shows that there is a difference in D and JH gene usage in functional rearrangements between lymphocytes from peripheral blood and spleen. Analysis of the VH6 gene shows that the mutational frequencies rise from 0.81% in cord blood to 1.96% in peripheral blood lymphocytes derived from young adults, and decrease to 0.80% in samples from individuals older than 50 years. The number of rearrangements carrying mutations follows a similar pattern: 22% in cord blood, 73% in the age group 20-49 years, and 57% in the age group over 50 years. The mutational frequencies among the mutated genes are, however, similar for cord blood and young adults, 2.76% and 2.51%, respectively, and 1.3% in older adults. These data show an age-related impaired affinity maturation which might relate to the decrease in immunological responsiveness among the elderly.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 1997. Vol. 27, no 6, 1381-1386 p.
Keyword [en]
senescence, affinity maturation, VH6 gene, CDR3, variability, ontogeny
National Category
Immunology in the medical area Medical Genetics
Research subject
Genetics; Immunology
Identifiers
URN: urn:nbn:se:umu:diva-80457OAI: oai:DiVA.org:umu-80457DiVA: diva2:649168
Available from: 2013-09-17 Created: 2013-09-17 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

No full text

Authority records BETA

Söderström, IngegerdHolmberg, Dan

Search in DiVA

By author/editor
Söderström, IngegerdHolmberg, Dan
By organisation
Department of Molecular Biology (Faculty of Medicine)
In the same journal
European Journal of Immunology
Immunology in the medical areaMedical Genetics

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 67 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf