Expression Studies of AminoAcid Transporters belonging to the Lysine and Hisitidine Transporter (LHT) Family in Hybrid Aspen Populus tremula L. x tremuloides Michx.
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
The human based input of fixed nitrogen, e.g. due to nitrogenous fertilizers, is the second most important driver of global change. The active input was, however, necessary due to a fast growing demand for agricultural products in order to feed an expanding world population in the last decades. Severe environmental damages are visible now, which is why it is crucial to find alternative ways to increase plant growth and biomass production without applying massive amounts of fertilizers. One way is to identify genes, which are able to improve nitrogen use efficiency (NUE) in plants when manipulated. Especially genes involved in nitrogen uptake, assimilation and remobilization, such as amino acid transporters are of great interest. Therefore a detailed knowledge about molecular processes regarding nitrogen transport in the respective plant species is crucial. So far, there is not much known about amino acid uptake mechanisms in tree species, which is why this work focuses on hybrid aspen. It was aimed to investigate the tissue expression patterns of genes encoding putative amino acid transporters in order to find potential target genes for improving NUE in the long term.
It was shown that eight homologs of a main Arabidopsis amino acid transporter, AtLHT1, are expressed in poplar. The eight amino acid transporters displayed different expression patterns, with expression in roots, stem and leaves of young hybrid aspen. To analyze the impacts of an increased amino acid uptake phenotype in a tree model system, PtLHT1.2 was cloned into an expression vector for Agrobacterium-mediated transformation into hybrid aspen. These results will be of great value for further studies regarding NUE in tree models.
Place, publisher, year, edition, pages
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-81461OAI: oai:DiVA.org:umu-81461DiVA: diva2:655914
Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU)
Sellstedt, Anita, ProfessorGaneteg, Ulrika, Forskare