(D)-amino acid chemical reporters reveal peptidoglycan dynamics of an intracellular pathogen
2013 (English)In: ACS Chemical Biology, ISSN 1554-8929, Vol. 8, no 3, 500-505 p.Article in journal (Refereed) Published
Peptidoglycan (PG) is an essential component of the bacterial cell wall. Although experiments with organisms in vitro have yielded a wealth of information on PG synthesis and maturation, it is unclear how these studies translate to bacteria replicating within host cells. We report a chemical approach for probing PG in vivo via metabolic labeling and bioorthogonal chemistry. A wide variety of bacterial species incorporated azide and alkyne-functionalized d-alanine into their cell walls, which we visualized by covalent reaction with click chemistry probes. The d-alanine analogues were specifically incorporated into nascent PG of the intracellular pathogen Listeria monocytogenes both in vitro and during macrophage infection. Metabolic incorporation of d-alanine derivatives and click chemistry detection constitute a facile, modular platform that facilitates unprecedented spatial and temporal resolution of PG dynamics in vivo.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2013. Vol. 8, no 3, 500-505 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-81857DOI: 10.1021/cb3004995PubMedID: 23240806OAI: oai:DiVA.org:umu-81857DiVA: diva2:658596