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In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids
Indiana University, Bloomington, USA.
Indiana University, Bloomington, USA.
Indiana University, Bloomington, USA.
Indiana University, Bloomington, USA.
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2012 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 51, no 50, 12519-12523 p.Article in journal (Refereed) Published
Abstract [en]

Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.

Place, publisher, year, edition, pages
2012. Vol. 51, no 50, 12519-12523 p.
Keyword [en]
bacteria, biosensors, D-amino acids, fluorescent probes, peptidoglycan
National Category
Chemical Sciences Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-81858DOI: 10.1002/anie.201206749ISI: 000312213800023PubMedID: 23055266OAI: oai:DiVA.org:umu-81858DiVA: diva2:658604
Available from: 2013-10-22 Created: 2013-10-22 Last updated: 2017-12-06Bibliographically approved

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