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An activity-independent selection system of thermostable protein variants
Biométhodes SA, Evry, France.
CBM ‘Severo Ochoa’ CSIC-UAM, Madrid, Spain.
Biométhodes SA, Evry, France.
Biométhodes SA, Evry, France.
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2007 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 4, no 11, 919-921 p.Article in journal (Refereed) Published
Abstract [en]

We describe an activity-independent method for the selection of thermostable mutants of any protein. It is based on a fusion construct comprising the protein of interest and a thermostable antibiotic resistance reporter, in such a way that thermostable mutants provide increased resistance in a thermophile. We isolated thermostable mutants of three human interferons and of two enzymes to demonstrate the applicability of the system.

Place, publisher, year, edition, pages
2007. Vol. 4, no 11, 919-921 p.
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:umu:diva-81869DOI: 10.1038/nmeth1090ISI: 000250575700015PubMedID: 17906633OAI: diva2:658618
Available from: 2013-10-22 Created: 2013-10-22 Last updated: 2013-10-28Bibliographically approved

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Cava, Felipe
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