An activity-independent selection system of thermostable protein variants
2007 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 4, no 11, 919-921 p.Article in journal (Refereed) Published
We describe an activity-independent method for the selection of thermostable mutants of any protein. It is based on a fusion construct comprising the protein of interest and a thermostable antibiotic resistance reporter, in such a way that thermostable mutants provide increased resistance in a thermophile. We isolated thermostable mutants of three human interferons and of two enzymes to demonstrate the applicability of the system.
Place, publisher, year, edition, pages
2007. Vol. 4, no 11, 919-921 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-81869DOI: 10.1038/nmeth1090ISI: 000250575700015PubMedID: 17906633OAI: oai:DiVA.org:umu-81869DiVA: diva2:658618