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Use of an antisense RNA strategy to investigate the functional significance of Mn-catalase in the extreme thermophile Thermus thermophilus
Centro de Biología Molecular 'Severo Ochoa' CSIC-UAM, Campus de Cantoblanco, Madrid, Spain.
Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Campus de Cantoblanco, Madrid, Spain.
Centro de Biología Molecular 'Severo Ochoa' CSIC-UAM, Campus de Cantoblanco, Madrid, Spain.
Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Campus de Cantoblanco, Madrid, Spain.
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2004 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 186, no 22, 7804-7806 p.Article in journal (Refereed) Published
Abstract [en]

The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive expression of a "bicistronic" transcript consisting of the kanamycin resistance gene mRNA followed by the antisense RNA against the selected target.

Place, publisher, year, edition, pages
2004. Vol. 186, no 22, 7804-7806 p.
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-81873DOI: 10.1128/JB.186.22.7804-7806.2004ISI: 000224973500039PubMedID: 15516595OAI: oai:DiVA.org:umu-81873DiVA: diva2:658625
Available from: 2013-10-22 Created: 2013-10-22 Last updated: 2017-12-06Bibliographically approved

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Cava, Felipe

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