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Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization
Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
Karolinska Institute, Södersjukhuset, Stockholm.
Karolinska University Hospital, Stockholm.
Umeå University, Faculty of Medicine, Department of Medical Biosciences, Medical and Clinical Genetics.
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2013 (English)In: Anaerobe, ISSN 1075-9964, E-ISSN 1095-8274, Vol. 23, 48-54 p.Article in journal (Refereed) Published
Abstract [en]

Propionibacterium acnes (P. acnes) and Propionibacterium granulosum (P. granulosum) are common skin colonizers that are implicated as possible contributing factors in acne vulgaris development. We have established direct visualization tools for the simultaneous detection of these closely related species with immunofluorescence assay and fluorescence in situ hybridization (FISH). As proof of principle, we were able to distinguish P. acnes and P. granulosum bacteria in multi-species populations in vitro as well as in a mock skin infection model upon labelling with 16S rRNA probes in combinatorial FISH as well as with antibodies. Furthermore, we report the co-localization of P. acnes and P. granulosum in the stratum corneum and hair follicles from patients with acne vulgaris as well as in healthy individuals. Further studies on the spatial distribution of these bacteria in skin structures in various skin disorders are needed.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 23, 48-54 p.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-82026DOI: 10.1016/j.anaerobe.2013.07.002PubMedID: 23896347OAI: oai:DiVA.org:umu-82026DiVA: diva2:659415
Available from: 2013-10-25 Created: 2013-10-25 Last updated: 2017-12-06Bibliographically approved

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Jahns, Anika CGolovleva, IrinaPalmer, Ruth HAlexeyev, Oleg A.
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PathologyMedical and Clinical GeneticsDepartment of Molecular Biology (Faculty of Science and Technology)
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CiteExportLink to record
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