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Essential roles of SHPS-1 in induction of contact hypersensitivity of skin.
Gunma University.
Gunma University.
Gunma University.
Gunma University.
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2008 (English)In: Immunology Letters, ISSN 0165-2478, E-ISSN 1879-0542, Vol. 121, no 1, 52-60 p.Article in journal (Refereed) Published
Abstract [en]

SHPS-1 is a transmembrane protein that binds the protein tyrosine phosphatases SHP-1 and SHP-2 and is abundant on the surface of CD11c(+) dendritic cells (DCs). We recently showed that SHPS-1 is essential for priming by DCs of CD4(+) T cells and for development of Th17 cell-mediated experimental autoimmunity. We have now further evaluated the importance of SHPS-1 and that of its ligand CD47 in contact hypersensitivity (CHS) to 2,4-dinitro-1-fluorobenzene (DNFB). Whereas the DNFB-induced CHS response was impaired in mice that express a mutant form of SHPS-1 lacking most of the cytoplasmic region, it was unaffected in CD47-deficient mice. Moreover, treatment of wild-type mice with mAbs to SHPS-1 that either block or do not block the binding of SHPS-1 to CD47 inhibited the CHS response. A mAb to CD47 had no such effect. The 2,4-dinitro-benzenesulfonic acid-induced proliferation of, and production of IFN-gamma or IL-17 by, T cells from DNFB-sensitized wild-type mice were inhibited by either mAb to SHPS-1 but not by that to CD47. In contrast, the blocking mAbs to SHPS-1, but not that to CD47, inhibited an allogeneic mixed leukocyte reaction. Both mAbs to SHPS-1, but not that to CD47, also inhibited the lipopolysaccharide- or polyinosinic-polycytidylic acid-induced production of TNF-alpha by DCs. These results suggest that SHPS-1 is essential for development of CHS, likely as a result of its positive regulation of the priming by DCs of CD4(+) T cells. However, such regulation by SHPS-1 does not appear to require its interaction with CD47.

Place, publisher, year, edition, pages
2008. Vol. 121, no 1, 52-60 p.
National Category
Basic Medicine
URN: urn:nbn:se:umu:diva-82570DOI: 10.1016/j.imlet.2008.08.005PubMedID: 18809435OAI: diva2:661913
Available from: 2013-11-05 Created: 2013-11-05 Last updated: 2013-11-05

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