Change search
ReferencesLink to record
Permanent link

Direct link
Selective increase of autoimmune epitope expression on aged erythrocytes in mice: implications in anti-erythrocyte autoimmune responses
University of Geneva.
University of Geneva.
University of Geneva.
Kyoto University.
Show others and affiliations
2002 (English)In: Journal of Autoimmunity, ISSN 0896-8411, E-ISSN 1095-9157, Vol. 18, no 1, 17-25 p.Article in journal (Refereed) Published
Abstract [en]

We investigated the impact of changes occurring during red blood cell (RBC) ageing on the RBC-binding activity of pathogenic anti-erythrocyte monoclonal antibodies derived from autoimmune-prone New Zealand black (NZB) mice. As assessed by flow cytometric analysis on in vivo biotinylated RBCs, all five NZB-derived anti-RBC mAb exhibited more efficient binding to aged RBCs than to young RBCs, and resulted in a selective elimination of more aged RBCs from the circulating blood. In addition, treatment of RBCs with proteases markedly enhanced the binding of all five anti-RBC mAb, raising the possibility that increased exposure of autoimmune epitopes on aged RBCs may be in part, a result of contacts with proteolytic enzymes during the lifetime of circulating RBCs. In marked contrast, the binding activity of mAb raised in non-autoimmune animals against antigens expressed on RBCs, such as CD44, CD47, CD147 and TER-119, was either decreased or unchanged with RBC ageing, and these epitopes, except for that recognized by anti-CD47 mAb, were highly sensitive to mild treatment with proteases. Our data unravel the unique molecular feature of RBC epitopes involved in autoimmune haemolytic anaemia, suggesting that membrane alterations in aged RBCs might play a significant role in the development of the autoantibody response to RBCs.

Place, publisher, year, edition, pages
Academic Press, 2002. Vol. 18, no 1, 17-25 p.
Keyword [en]
autoantibody, autoantigen, autoimmune haemolytic anaemia, aging
National Category
Immunology in the medical area
URN: urn:nbn:se:umu:diva-82577DOI: 10.1006/jaut.2001.0563ISI: 000174501100003PubMedID: 11869043OAI: diva2:661923
Available from: 2013-11-05 Created: 2013-11-05 Last updated: 2014-04-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Oldenborg, Per-Arne
By organisation
Histology and Cell Biology
In the same journal
Journal of Autoimmunity
Immunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 19 hits
ReferencesLink to record
Permanent link

Direct link