Isolation of monomeric photosystem II that retains the subunit PsbS.
2013 (English)In: Photosynthesis Research, ISSN 0166-8595, E-ISSN 1573-5079, Vol. 118, no 3, 199-207 p.Article in journal (Refereed) Published
Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.
Place, publisher, year, edition, pages
Springer, 2013. Vol. 118, no 3, 199-207 p.
IdentifiersURN: urn:nbn:se:umu:diva-82591DOI: 10.1007/s11120-013-9914-2PubMedID: 23975205OAI: oai:DiVA.org:umu-82591DiVA: diva2:662017