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Adsorption of proteins involved in hydrolysis of lignocellulose on lignins and hemicelluloses
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2013 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 148, 70-77 p.Article in journal (Refereed) Published
Abstract [en]

Protein adsorption onto eight lignocellulosic substances (six lignin preparations and two hemicelluloses) was investigated at pH 4.8 and at two different temperatures (4°C and 45°C). The kinetics of the adsorption of cellulase, xylanase, and β-glucosidase were determined by enzyme activity measurements. The maximum adsorption capacities, the affinity constants and the binding strengths varied widely and were typically higher for the lignins than for the carbohydrates. As indicated by BET and gel permeation chromatography, different substances had widely different surface area, pore size, weight average molecular weight, and polydispersity index, but these properties were difficult to relate to protein binding. In most cases, an increase in temperature from 4°C to 45°C and a low content of carboxylic acid groups, as indicated by Fourier-Transform Infra-Red (FTIR) spectroscopy, resulted in increased protein adsorption capacity, which suggests that hydrophobic interactions play an important role.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 148, 70-77 p.
Keyword [en]
ionic liquid, 1-allyl-3-methylimidazolium formate, lignocellulose; pretreatment, enzymatic hydrolysis
National Category
Chemical Sciences Agricultural Sciences
URN: urn:nbn:se:umu:diva-82599DOI: 10.1016/j.biortech.2013.08.121PubMedID: 24045193OAI: diva2:662029
Swedish Research Council, 621-2011-4388Swedish Energy Agency, P35367-1Bio4Energy
Available from: 2013-11-05 Created: 2013-11-05 Last updated: 2013-12-11Bibliographically approved

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Pareek, NidhiGillgren, ThomasJönsson, Leif J
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