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A composite poly-hydroxybutyrate-glial growth factor conduit for long nerve gap repairs
Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Hand Surgery. University of Manchester.
University of Manchester.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy. Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Hand Surgery.
University of Manchester.
2003 (English)In: Journal of Anatomy, ISSN 0021-8782, E-ISSN 1469-7580, Vol. 203, no 6, 553-565 p.Article in journal (Refereed) Published
Abstract [en]

There is considerable evidence that peripheral nerves have the potential to regenerate in an appropriate microenvironment. We have developed a novel artificial nerve guide composed of poly 3-hydroxybutyrate (PHB) filled with glial growth factor (GGF) suspended in alginate hydrogel. Gaps of 2-4 cm in rabbit common peroneal nerve were bridged using a PHB conduit containing either GGF in alginate hydrogel (GGF) or alginate alone (Alginate), or with an empty PHB conduit (Empty). Tissues were harvested 21, 42 and 63 days post-operatively. Schwann cell and axonal regeneration were assessed using quantitative immunohistochemistry. At 21 days, addition of GGF increased significantly the distance of axonal and Schwann cells regeneration in comparison with that observed in Alginate and Empty conduits for both gap lengths. The axons bridged the 2-cm GGF conduits gap by 63 days, with a comparable rate of regeneration seen in 4-cm conduits. Schwann cells and axonal regeneration quantity was similar for both gap lengths in each group. However, at all time points the quantity of axonal and Schwann cells regeneration in GGF grafts was significantly greater than in both Alginate and Empty conduits, the latter showing better regeneration than Alginate conduits. The results indicate an inhibitory effect of alginate on regeneration, which is partially reversed by the addition of GGF to the conduits. In conclusion, GGF stimulates a progressive and sustainable regeneration increase in long nerve gap conduits.

Place, publisher, year, edition, pages
Blackwell Publishing, 2003. Vol. 203, no 6, 553-565 p.
Keyword [en]
alginate, gap repair, glial growth factors, nerve bioengineering, nerve injury, peripheral nervous system, regeneration, Schwann cells
National Category
Neurosciences Neurology
Identifiers
URN: urn:nbn:se:umu:diva-82671DOI: 10.1046/j.1469-7580.2003.00243.xISI: 000187108400002PubMedID: 14686691OAI: oai:DiVA.org:umu-82671DiVA: diva2:662136
Available from: 2013-11-06 Created: 2013-11-06 Last updated: 2017-12-06Bibliographically approved

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