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Human skeletal muscle satellite cells: aging, oxidative stress and the mitotic clock
UMR CNRS 7000, Cytosquelette et Développement, Faculté de Medecine Pitié-Salpétrière, 105, bd de l'Hôpital, F-75634 Paris Cedex 13, France.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
UMR CNRS 7000, Cytosquelette et Développement, Faculté de Medecine Pitié-Salpétrière, 105, bd de l'Hôpital, F-75634 Paris Cedex 13, France.
UMR CNRS 7000, Cytosquelette et Développement, Faculté de Medecine Pitié-Salpétrière, 105, bd de l'Hôpital, F-75634 Paris Cedex 13, France.
2002 (English)In: Experimental Gerontology, ISSN 0531-5565, E-ISSN 1873-6815, Vol. 37, no 10-11, 1229-1236, Article Number: PII S0531-5565(02)00129-8 p.Article in journal (Refereed) Published
Abstract [en]

Normal satellite cell cultures, isolated from human skeletal muscle, have a limited proliferative capacity and inevitably reach replicative senescence. In this study, we have focused on the consequences of a single oxidative stress by hydrogen peroxide (H(2)O(2)) on both proliferative capacity and myogenic characteristics. Treatment with 1mM H(2)O(2) for 30 min causes a small decrease in the viability and lifespan while the number of cells which are able to proliferate, decreases dramatically. This premature arrest of the cells in a non-proliferative state was not due to spontaneous differentiation since there was no increase in the number of myogenin positive cells. This stress did not affect the myogenicity of the cells or their ability to differentiate and fuse to form multinucleated myotubes. In addition, the mitotic clock does not seem to be modified by oxidative stress treatment since the rate of telomere shortening was similar in H(2)O(2)-treated and control cells. This could be the consequence of the high level of oxygen consumption with an even higher level of ROS being produced in skeletal muscle than in other tissues which would be counteracted by an increase in the antioxidant defense system.

Place, publisher, year, edition, pages
Elsevier, 2002. Vol. 37, no 10-11, 1229-1236, Article Number: PII S0531-5565(02)00129-8 p.
Keyword [en]
satellite cell, muscle, senescence, oxidative stress, H2O2, telomere, aging, human
National Category
Geriatrics
Identifiers
URN: urn:nbn:se:umu:diva-82686DOI: 10.1016/S0531-5565(02)00129-8ISI: 000179722100013PubMedID: 12470836OAI: oai:DiVA.org:umu-82686DiVA: diva2:662165
Conference
Conference: 2nd Euroconference on Biological Ageing, Location: Spetses Isl, Greece, Date: May 18-22, 2002
Available from: 2013-11-06 Created: 2013-11-06 Last updated: 2017-12-06Bibliographically approved

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