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Localization of laminin alpha4-chain in developing and adult human tissues
University of Helsinki.
Helsinki University Central Hospital.
Karolinska Institute and BioStratum AB, Stockholm.
Karolinska Institute.
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2002 (English)In: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 50, no 8, 1113-1130 p.Article in journal (Refereed) Published
Abstract [en]

Recent studies suggest important functions for laminin-8 (Ln-8; alpha4beta1gamma1) in vascular and blood cell biology, but its distribution in human tissues has remained elusive. We have raised a monoclonal antibody (MAb) FC10, and by enzyme-linked immunoassay (EIA) and Western blotting techniques we show that it recognizes the human Ln alpha4-chain. Immunoreactivity for the Ln alpha4-chain was localized in tissues of mesodermal origin, such as basement membranes (BMs) of endothelia, adipocytes, and skeletal, smooth, and cardiac muscle cells. In addition, the Ln alpha4-chain was found in regions of some epithelial BMs, including epidermis, salivary glands, pancreas, esophageal and gastric glands, intestinal crypts, and some renal medullary tubules. Developmental differences in the distribution of Ln alpha4-chain were detected in skeletal muscle, walls of vessels, and intestinal crypts. Ln alpha4- and Ln alpha2-chains co-localized in BMs of fetal skeletal muscle cells and in some epithelial BMs, e.g., in gastric glands and acini of pancreas. Cultured human pulmonary artery endothelial (HPAE) cells produced Ln alpha4-chain as M(r) 180,000 and 200,000 doublet and rapidly deposited it to the growth substratum. In cell-free extracellular matrices of human kidney and lung, Ln alpha4-chain was found as M(r) 180,000 protein.

Place, publisher, year, edition, pages
2002. Vol. 50, no 8, 1113-1130 p.
Keyword [en]
basement membrane, laminin-8, alpha 4-chain, monoclonal antibody, endothelial cell
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-82687DOI: 10.1177/002215540205000813ISI: 000177068700013PubMedID: 12133914OAI: diva2:662167
Available from: 2013-11-06 Created: 2013-11-06 Last updated: 2014-04-16Bibliographically approved

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