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Efficiency of photosynthetic water oxidation at ambient and depleted levels of inorganic carbon
Umeå University, Faculty of Science and Technology, Department of Chemistry. Max Planck Institute for Chemical Energy Conversion, Mülheim, Germany.
Max Planck Institute for Chemical Energy Conversion, Mülheim, Germany.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
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2013 (English)In: Photosynthesis Research, ISSN 0166-8595, E-ISSN 1573-5079, Vol. 117, no 1-3, Special Issues on Photosynthesis Education Honoring Govindjee, 401-412 p.Article in journal (Refereed) Published
Abstract [en]

Over 40 years ago, Joliot et al. (Photochem Photobiol 10:309-329, 1969) designed and employed an elegant and highly sensitive electrochemical technique capable of measuring O2 evolved by photosystem II (PSII) in response to trains of single turn-over light flashes. The measurement and analysis of flash-induced oxygen evolution patterns (FIOPs) has since proven to be a powerful method for probing the turnover efficiency of PSII. Stemler et al. (Proc Natl Acad Sci USA 71(12):4679-4683, 1974), in Govindjee's lab, were the first to study the effect of "bicarbonate" on FIOPs by adding the competitive inhibitor acetate. Here, we extend this earlier work by performing FIOPs experiments at various, strictly controlled inorganic carbon (Ci) levels without addition of any inhibitors. For this, we placed a Joliot-type bare platinum electrode inside a N2-filled glove-box (containing 10-20 ppm CO2) and reduced the Ci concentration simply by washing the samples in Ci-depleted media. FIOPs of spinach thylakoids were recorded either at 20-times reduced levels of Ci or at ambient Ci conditions (390 ppm CO2). Numerical analysis of the FIOPs within an extended Kok model reveals that under Ci-depleted conditions the miss probability is discernibly larger (by 2-3 %) than at ambient conditions, and that the addition of 5 mM HCO3 (-) to the Ci-depleted thylakoids largely restores the original miss parameter. Since a "mild" Ci-depletion procedure was employed, we discuss our data with respect to a possible function of free or weakly bound HCO3 (-) at the water-splitting side of PSII.

Place, publisher, year, edition, pages
Springer, 2013. Vol. 117, no 1-3, Special Issues on Photosynthesis Education Honoring Govindjee, 401-412 p.
Keyword [en]
Flash-induced oxygen evolution patterns, S states, An extended Kok model, Hydrogen carbonate (bicarbonate), Photosynthetic water oxidation
National Category
Chemical Sciences Biochemistry and Molecular Biology Botany
URN: urn:nbn:se:umu:diva-83168DOI: 10.1007/s11120-013-9875-5PubMedID: 23828399OAI: diva2:665638
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Available from: 2013-11-20 Created: 2013-11-20 Last updated: 2015-04-29Bibliographically approved
In thesis
1. Water splitting in natural and artificial photosynthetic systems
Open this publication in new window or tab >>Water splitting in natural and artificial photosynthetic systems
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Photosynthesis is the unique biological process that converts carbon dioxide into organic compounds, for example sugars, using the energy of sunlight. Thereby solar energy is converted into chemical energy. Nearly all life depends on this reaction, either directly, or indirectly as the ultimate source of their food. Oxygenic photosynthesis occurs in plants, algae and cyanobacteria. This process created the present level of oxygen in the atmosphere, which allowed the formation of higher life, since respiration allows extracting up to 15-times more energy from organic matter than anaerobic fermentation. Oxygenic photosynthesis uses as substrate for the ubiquitous water. The light-induced oxidation of water to molecular oxygen (O2), catalyzed by the Mn4CaO5 cluster associated with the photosystem II (PS II) complex, is thus one of the most important and wide spread chemical processes occurring in the biosphere. Understanding the mechanism of water-oxidation by the Mn4CaO5 cluster is one of today’s great challenges in science. It is believed that one can extract basic principles of catalyst design from the natural system that than can be applied to artificial systems. Such systems can be used in the future for the generation of fuel from sunlight.

In this thesis the light-induced production of molecular oxygen and carbon dioxide (CO2) by PSII was observed by membrane-inlet mass spectrometry. By analyzing this observation is shown that CO2 not only is the substrate in photosynthesis for the production of sugars, but that it also regulates the efficiency of the initial steps of the electron transport chain of oxygenic photosynthesis by acting, in form of HCO3-, as acceptor for protons produced during water-splitting. This finding concludes the 50-years old search for the function of CO2/HCO3 in photosynthetic water oxidation.

For understanding the mechanism of water oxidation it is crucial to resolve the structures of all oxidation states, including transient once, of the Mn4CaO5 cluster. With this application in mind a new illumination setup was developed and characterized that allowed to bring the Mn4CaO5 cluster of PSII microcrystals into known oxidation states while they flow through a narrow capillary. The optimized illumination conditions were employed at the X-ray free electron laser at the Linac Coherent Light Source (LCLS) to obtain simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) at room temperature. This two methods probe the overall protein structure and the electronic structure of the Mn4CaO5 cluster, respectively. Data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. This approach opens new directions for studying structural changes during the catalytic cycle of the Mn4CaO5 cluster, and for resolving the mechanism of O-O bond formation.

In two other projects the mechanism of molecular oxygen formation by artificial water oxidation catalysts containing inexpensive and abundant elements were studied. Oxygen evolution catalyzed by calcium manganese and manganese only oxides was studied in 18O-enriched water. It was concluded that molecular oxygen is formed by entirely different pathways depending on what chemical oxidant was used.  Only strong non-oxygen donating oxidants were found to support ‘true’ water-oxidation. For cobalt oxides a study was designed to understand the mechanistic details of how the O-O bond forms. The data demonstrate that O-O bond formation occurs by direct coupling between two terminal water-derived ligands. Moreover, by detailed theoretical modelling of the data the number of cobalt atoms per catalytic site was derived.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2014. 96 p.
Water splitting, photosystem II, artificial catalyst, MIMS, inorganic carbon
National Category
Chemical Sciences Biophysics
urn:nbn:se:umu:diva-86363 (URN)978-91-7459-800-1 (ISBN)
Public defence
2014-03-21, KB3B3, stora hörsalen, KBC-huset, Umeå, 13:00 (English)
Available from: 2014-02-28 Created: 2014-02-24 Last updated: 2014-02-27Bibliographically approved

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Shevela, DmitriyKoroidov, SergeyShutova, TatyanaSamuelsson, GöranMessinger, Johannes
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