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Quantification of melatonin in human saliva by liquid chromatography-tandem mass spectrometry using stable isotope dilution
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Occupational and Environmental Medicine.
National Institute for Working Life, Umeå.
National Institute for Working Life, Umeå.
2003 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 794, no 1, 115-123 p.Article in journal (Refereed) Published
Abstract [en]

A method for the determination of melatonin in human saliva has been developed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS). Saliva was collected in plastic tubes. 7-D-Melatonin was added as internal standard and the samples were cleaned and concentrated by solid-phase extraction. The limit of detection was 1.05 pg x ml(-1) and the limit of quantification was 3.0 pg x ml(-1). The accuracy of the method was +/-14% at 5.60 pg x ml(-1) and +/-9% at 19.6 pg x ml(-1). The precision was +/-13% at 6.18 pg x ml(-1) and +/-11% at 31.2 pg x ml(-1), respectively. Our HPLC-MS-MS method shows a high sensitivity and specificity for melatonin and more reliable results compared with a radioimmunoassay. The chromatographic method has been used to determine the circadian rhythm of melatonin among three nurses working the night shift and a patient suffering from an inability to fall asleep at night.

Place, publisher, year, edition, pages
Elsevier, 2003. Vol. 794, no 1, 115-123 p.
Keyword [en]
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:umu:diva-83460DOI: 10.1016/S1570-0232(03)00425-2ISI: 000184526000013PubMedID: 12888204OAI: diva2:667410
Available from: 2013-11-26 Created: 2013-11-26 Last updated: 2014-04-11Bibliographically approved

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Eriksson, KåreLevin, Jan-Olof
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