Quantification of melatonin in human saliva by liquid chromatography-tandem mass spectrometry using stable isotope dilution
2003 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 794, no 1, 115-123 p.Article in journal (Refereed) Published
A method for the determination of melatonin in human saliva has been developed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS). Saliva was collected in plastic tubes. 7-D-Melatonin was added as internal standard and the samples were cleaned and concentrated by solid-phase extraction. The limit of detection was 1.05 pg x ml(-1) and the limit of quantification was 3.0 pg x ml(-1). The accuracy of the method was +/-14% at 5.60 pg x ml(-1) and +/-9% at 19.6 pg x ml(-1). The precision was +/-13% at 6.18 pg x ml(-1) and +/-11% at 31.2 pg x ml(-1), respectively. Our HPLC-MS-MS method shows a high sensitivity and specificity for melatonin and more reliable results compared with a radioimmunoassay. The chromatographic method has been used to determine the circadian rhythm of melatonin among three nurses working the night shift and a patient suffering from an inability to fall asleep at night.
Place, publisher, year, edition, pages
Elsevier, 2003. Vol. 794, no 1, 115-123 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-83460DOI: 10.1016/S1570-0232(03)00425-2ISI: 000184526000013PubMedID: 12888204OAI: oai:DiVA.org:umu-83460DiVA: diva2:667410