umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Laminin chains in developing and adult human myotendinous junctions.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Department of Cell & Molecular Biology, Uppsala University, Uppsala.
Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Show others and affiliations
2000 (English)In: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 48, no 2, 201-10 p.Article in journal (Refereed) Published
Abstract [en]

In addition to being the specialized site for transmission of force from the muscle to the tendon, the myotendinous junction (MTJ) also plays an important role in muscle splitting during morphogenesis. An early event in the formation of the MTJ is a regional deposition of basement membranes. We used immunocytochemistry to investigate the distribution of laminin chains during the development of MTJs in human limb muscle at 8-22 weeks of gestation (wg) and in adult MTJs. We used polyclonal antibodies and a new monoclonal antibody (MAb) against the human laminin alpha1 G4/G5 domains. At 8-10 wg, laminin alpha1 and laminin alpha5 chains were specifically localized to the MTJ. Laminin alpha1 chain remained restricted to the MTJ at 22 wg as the laminin beta2 chain had appeared, whereas the laminin alpha5 chain became deposited along the entire length of the myotubes from 12 wg. In the adult MTJ, only vestigial amounts of laminin alpha1 and laminin alpha5 chains could be detected. On the basis of co-distribution data, we speculate that laminin alpha1 chain in the forming MTJ undergoes an isoform switch from laminin 1 to laminin 3. Our data indicate a potentially important role for laminin alpha1 chain in skeletal muscle formation.

Place, publisher, year, edition, pages
2000. Vol. 48, no 2, 201-10 p.
Keyword [en]
myotendinous junction, laminin alpha 1 chain, development, human, muscle
National Category
Basic Medicine
Identifiers
URN: urn:nbn:se:umu:diva-83536PubMedID: 10639486OAI: oai:DiVA.org:umu-83536DiVA: diva2:668672
Available from: 2013-12-02 Created: 2013-12-02 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

No full text

PubMed

Authority records BETA

Pedrosa-Domellöf, FatimaThornell, L E

Search in DiVA

By author/editor
Pedrosa-Domellöf, FatimaThornell, L E
By organisation
Anatomy
In the same journal
Journal of Histochemistry and Cytochemistry
Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 46 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf