A 3D in vitro model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury
2013 (English)In: Regenerative Medicine, ISSN 1746-0751, Vol. 8, no 6, 739-746 p.Article in journal (Refereed) Published
AIM: This study aimed to develop a 3D culture model to test the extent to which transplanted stem cells modulate astrocyte reactivity, where exacerbated glial cell activation could be detrimental to CNS repair success.
MATERIALS & METHODS: The reactivity of rat astrocytes to bone marrow mesenchymal stem cells, neural crest stem cells (NCSCs) and differentiated adipose-derived stem cells was assessed after 5 days. Schwann cells were used as a positive control.
RESULTS: NCSCs and differentiated Schwann cell-like adipose-derived stem cells did not increase astrocyte reactivity. Highly reactive responses to bone marrow mesenchymal stem cells and Schwann cells were equivalent.
CONCLUSION: This approach can screen therapeutic cells prior to in vivo testing, allowing cells likely to trigger a substantial astrocyte response to be identified at an early stage. NCSCs and differentiated Schwann cell-like adipose-derived stem cells may be useful in treating CNS damage without increasing astrogliosis.
Place, publisher, year, edition, pages
Future Medicine Ltd. , 2013. Vol. 8, no 6, 739-746 p.
3D cell culture, astrocytes, CNS, reactive gliosis, spinal cord, stem cell therapy
Neurosciences Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:umu:diva-83709DOI: 10.2217/rme.13.61PubMedID: 24147529OAI: oai:DiVA.org:umu-83709DiVA: diva2:675973
FunderSwedish Research CouncilWellcome trust, 080309