Integrated biological-chemical approach for the isolation and selection of polyaromatic mutagens in surface waters
2013 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 405, no 28, 9101-9112 p.Article in journal (Refereed) Published
Many environmental mutagens, including polyaromatic compounds are present in surface waters, often in complex mixtures and at low concentrations. The present study provides and applies a novel, integrated approach to isolate polyaromatic mutagens in river water using a sample from the River Elbe. The sample was taken downstream of industrial discharges using blue rayon (BR) as a passive sampler that selectively adsorbs polyaromatic compounds and was subjected to effect-directed fractionation in order to characterise the compounds causing the detected effect(s). The procedure relies on three complementary fractionation steps, the Ames fluctuation assay with strains TA98, YG1024 and YG1041 with and without S9 activation and analytical screening. Several mutagenic fractions were isolated by combining mutagenicity testing with fractionation. The enhanced mutagenicity in the nitroreductase and/or O-acetyltransferase overexpressing strains YG1024 and YG1041 strains suggested amino- and/or nitro-compounds causing mutagenicity in several fractions. Analytical screening of mutagenic fractions with LC-HRMS/MS provided a list of molecular formulas typically containing one to ten nitrogen and at least two oxygen atoms supporting the presence of amino and nitro-compounds in the mutagenic fractions.
Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2013. Vol. 405, no 28, 9101-9112 p.
Polyaromatic compounds, Blue rayon, Multi-dimensional fractionation, Multi-strains Ames fluctuation assay, Reversed-phase HPLC, Liquid-chromatography-high resolution MS/MS
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-83617DOI: 10.1007/s00216-013-7349-4ISI: 000326374100015OAI: oai:DiVA.org:umu-83617DiVA: diva2:676335