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Detecting conserved regulatory elements with the model genome of the Japanese puffer fish, Fugu rubripes.
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience, Clinical Neuroscience.
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1995 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 92, no 5, 1684-1688 p.Article in journal (Refereed) Published
Abstract [en]

Comparative vertebrate genome sequencing offers a powerful method for detecting conserved regulatory sequences. We propose that the compact genome of the teleost Fugu rubripes is well suited for this purpose. The evolutionary distance of teleosts from other vertebrates offers the maximum stringency for such evolutionary comparisons. To illustrate the comparative genome approach for F. rubripes, we use sequence comparisons between mouse and Fugu Hoxb-4 noncoding regions to identify conserved sequence blocks. We have used two approaches to test the function of these conserved blocks. In the first, homologous sequences were deleted from a mouse enhancer, resulting in a tissue-specific loss of activity when assayed in transgenic mice. In the second approach, Fugu DNA sequences showing homology to mouse sequences were tested for enhancer activity in transgenic mice. This strategy identified a neural element that mediates a subset of Hoxb-4 expression that is conserved between mammals and teleosts. The comparison of noncoding vertebrate sequences with those of Fugu, coupled to a transgenic bioassay, represents a general approach suitable for many genome projects.

Place, publisher, year, edition, pages
1995. Vol. 92, no 5, 1684-1688 p.
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Biological Sciences
URN: urn:nbn:se:umu:diva-84181DOI: 10.1073/pnas.92.5.1684ISI: A1995QK07700090PubMedID: 7878040OAI: diva2:679864
Available from: 2013-12-16 Created: 2013-12-16 Last updated: 2014-02-17Bibliographically approved

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Aparicio, SMorrison, AGould, AGilthorpe, JonathanChaudhuri, CRigby, PKrumlauf, RBrenner, S
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