Change search
ReferencesLink to record
Permanent link

Direct link
Aberrant VHGene Utilization in Patients with Established Insulin-Dependent Diabetes Mellitus
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Medicine.
Show others and affiliations
1997 (English)In: Journal of Autoimmunity, ISSN 0896-8411, E-ISSN 1095-9157, Vol. 10, no 2, 157-163 p.Article in journal (Refereed) Published
Abstract [en]

We have compared the B-lymphocyte repertoire in seven IDDM patients with 12 healthy controls by examining the variable heavy (VH) gene expression. The VHgene representation in the pool of pokeweed mitogen (PWM) stimulated, immunocompetent B cells and in the pool of naturally activated plasma cells (actual repertoire) was analysed by RNA-RNA in situ hybridization. Differences between IDDM patients and normal controls in the relative expression of several VHgene families were observed. In IDDM patients, the VH3 was significantly underrepresented in the PWM stimulated repertoire. In the actual B cell repertoire the VH5 clones were underrepresented among diabetic patients. Moreover, the altered distribution of VHgene usage between the PWM stimulated repertoire and the actual repertoire observed in normal controls was found to be less pronounced in the IDDM patients. This observation suggests a defect in the V-gene directed cellular selection occurring between resting, immunocompetent B cells and naturally activated plasma cells. The possible implication of the observed aberrations in the B cell selection process for the pathogenesis of autoimmunity is discussed.

Place, publisher, year, edition, pages
1997. Vol. 10, no 2, 157-163 p.
Keyword [en]
B cell repertoire, IDDM, VHgene family
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-84187ISI: A1997XA35100006PubMedID: 9185877OAI: diva2:679919
Available from: 2013-12-17 Created: 2013-12-17 Last updated: 2014-02-17Bibliographically approved

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Hillörn, ValterSöderström, IngegerdCilio, CorradoForsgren, StinaHägg, ErikLundkvist, ingerHolmberg, Dan
By organisation
MedicineClinical Immunology
In the same journal
Journal of Autoimmunity
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 35 hits
ReferencesLink to record
Permanent link

Direct link