Resistance to low temperature photoinhibition is not associated with isolated thylakoid membranes of winter rye.
1991 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 97, no 2, 804-810 p.Article in journal (Refereed) Published
In vivo measurements of chlorophyll a fluorescence indicate that cold-hardened winter rye (Secale cereale L. cv Musketeer) develops a resistance to low temperature-induced photoinhibition compared with nonhardened rye. After 7.2 hours at 5 degrees C and 1550 micromoles per square meter per second, the ratio of variable fluorescence/maximum fluorescence was depressed by only 23% in cold-hardened rye compared with 46% in nonhardened rye. We have tested the hypothesis that the principal site of this resistance to photoinhibition resides at the level of rye thylakoid membranes. Thylakoids were isolated from cold-hardened and nonhardened rye and exposed to high irradiance (1000-2600 micromoles per square meter per second) at either 5 or 20 degrees C. The photoinhibitory response measured by room temperature fluorescence induction, photosystem II electron transport, photoacoustic spectroscopy, or [(14)C]atrazine binding indicates that the differential resistance to low temperature-induced photoinhibition in vivo is not observed in isolated thylakoids. Similar results were obtained whether isolated rye thylakoids were photoinhibited or thylakoids were isolated from rye leaves preexposed to a photoinhibitory treatment. Thus, we conclude that increased resistance to low temperature-induced photoinhibition is not a property of thylakoid membranes but is associated with a higher level of cellular organization.
Place, publisher, year, edition, pages
1991. Vol. 97, no 2, 804-810 p.
Research subject Physiological Botany
IdentifiersURN: urn:nbn:se:umu:diva-84220DOI: 10.1104/pp.97.2.804ISI: A1991GM05700048PubMedID: 16668470OAI: oai:DiVA.org:umu-84220DiVA: diva2:680827