Canonical Wnt/beta-Catenin Signalling Is Essential for Optic Cup Formation
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 12, e81158- p.Article in journal (Refereed) Published
A multitude of signalling pathways are involved in the process of forming an eye. Here we demonstrate that beta-catenin is essential for eye development as inactivation of beta-catenin prior to cellular specification in the optic vesicle caused anophthalmia in mice. By achieving this early and tissue-specific beta-catenin inactivation we find that retinal pigment epithelium (RPE) commitment was blocked and eye development was arrested prior to optic cup formation due to a loss of canonical Wnt signalling in the dorsal optic vesicle. Thus, these results show that Wnt/beta-catenin signalling is required earlier and play a more central role in eye development than previous studies have indicated. In our genetic model system a few RPE cells could escape beta-catenin inactivation leading to the formation of a small optic rudiment. The optic rudiment contained several neural retinal cell classes surrounded by an RPE. Unlike the RPE cells, the neural retinal cells could be beta-catenin- negative revealing that differentiation of the neural retinal cell classes is beta-catenin-independent. Moreover, although dorsoventral patterning is initiated in the mutant optic vesicle, the neural retinal cells in the optic rudiment displayed almost exclusively ventral identity. Thus, beta-catenin is required for optic cup formation, commitment to RPE cells and maintenance of dorsal identity of the retina.
Place, publisher, year, edition, pages
Public Library of Science , 2013. Vol. 8, no 12, e81158- p.
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-85289DOI: 10.1371/journal.pone.0081158ISI: 000327949300074OAI: oai:DiVA.org:umu-85289DiVA: diva2:694086
FunderSwedish Research CouncilSwedish Cancer Society