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Canonical Wnt/beta-Catenin Signalling Is Essential for Optic Cup Formation
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).
2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 12, e81158- p.Article in journal (Refereed) Published
Abstract [en]

A multitude of signalling pathways are involved in the process of forming an eye. Here we demonstrate that beta-catenin is essential for eye development as inactivation of beta-catenin prior to cellular specification in the optic vesicle caused anophthalmia in mice. By achieving this early and tissue-specific beta-catenin inactivation we find that retinal pigment epithelium (RPE) commitment was blocked and eye development was arrested prior to optic cup formation due to a loss of canonical Wnt signalling in the dorsal optic vesicle. Thus, these results show that Wnt/beta-catenin signalling is required earlier and play a more central role in eye development than previous studies have indicated. In our genetic model system a few RPE cells could escape beta-catenin inactivation leading to the formation of a small optic rudiment. The optic rudiment contained several neural retinal cell classes surrounded by an RPE. Unlike the RPE cells, the neural retinal cells could be beta-catenin- negative revealing that differentiation of the neural retinal cell classes is beta-catenin-independent. Moreover, although dorsoventral patterning is initiated in the mutant optic vesicle, the neural retinal cells in the optic rudiment displayed almost exclusively ventral identity. Thus, beta-catenin is required for optic cup formation, commitment to RPE cells and maintenance of dorsal identity of the retina.

Place, publisher, year, edition, pages
Public Library of Science , 2013. Vol. 8, no 12, e81158- p.
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-85289DOI: 10.1371/journal.pone.0081158ISI: 000327949300074OAI: oai:DiVA.org:umu-85289DiVA: diva2:694086
Funder
Swedish Research CouncilSwedish Cancer Society
Available from: 2014-02-05 Created: 2014-01-31 Last updated: 2017-12-06Bibliographically approved

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Hägglund, Anna-CarinBerghard, Anna

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