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Synthetic crowding agent dextran causes excluded volume interactions exclusively to tracer protein apoazurin
Umeå University, Faculty of Science and Technology, Department of Chemistry. (Pernilla Wittungs-Stafshede)
Umeå University, Faculty of Science and Technology, Department of Chemistry. (Biological Chemistry)
2014 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 588, no 5, 811-814 p.Article in journal (Refereed) Published
Abstract [en]

To understand protein biophysics in crowded cellular environments, researchers often use synthetic polymers as 'crowding agents' in vitro. The idea is that these agents will occupy space and reproduce the in vivo scenario in terms of excluded volume. However, recent work has challenged this concept and pointed out that attractive interactions between protein and crowding agent will provide an enthalpic contribution to the overall effect on protein thermodynamics. Here we use a typical synthetic crowding agent and a well-studied model protein to demonstrate in a window of 50 K that the presence of dextran 20 affects apoazurin by steric repulsion. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 588, no 5, 811-814 p.
Keyword [en]
Macromolecular crowding, Crowding agent, Excluded volume, Attractive interaction, Spectroscopy, Azurin
National Category
Chemical Sciences
URN: urn:nbn:se:umu:diva-87389DOI: 10.1016/j.febslet.2014.01.043ISI: 000331717500026OAI: diva2:709338
Available from: 2014-04-01 Created: 2014-03-31 Last updated: 2014-04-01Bibliographically approved

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Christiansen, AlexanderWittung-Stafshede, Pernilla
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