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Analysis of VH gene utilisation in the non-obese diabetic mouse
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Department of Cell and Molecular Biology. University of Umeå)
Unite d'lmmunobiologie, lnsritut Pasteur, Paris, France.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Department of Cell and Molecular Biology. University of Umeå)
1993 (English)In: Autoimmunity, ISSN 0891-6934, E-ISSN 1607-842X, Vol. 15, no 1, 11-18 p.Article in journal (Refereed) Published
Abstract [en]

The immunoglobulin (Ig) heavy chain variable (VH) gene complexity and the VH gene utilisation pattern of the non-obese diabetic (NOD) mouse were investigated. We found that the NOD mouse displays a VH gene complexity which appears to be identical to that of the C57BL/6 mouse. Thus, Southern hybridisation using probes specific for 9 of the murine VH gene families revealed identical restriction fragment length polymorphism (RFLP) patterns in both mouse strains. As indicated by immunofluorescence analysis using allotype specific monoclonal antibodies the NOD mice were also found to carry the IgCH-1b allele. Collectively, these data suggest that the NOD mice carry an IgVH locus identical to that carried by C57BL/6. In contrast to the apparent identity at the level of germline VH gene repertoires, the pattern of VH gene utilisation differed considerably between these two mouse strains. Thus, in NOD mice the neonatal preference of D-proximal VH genes was found to be more pronounced than in C57BL/6 mice. Moreover, in contrast to adult C57BL/6 mice a D-proximal bias was evident also in adult NOD mice. On the basis of these findings we discuss the possibility that the distorted development of B cell repertoires in the NOD mouse could be directly or indirectly related to the T cell mediated, autoimmune process in the NOD mouse.

Place, publisher, year, edition, pages
Harwood Academic, 1993. Vol. 15, no 1, 11-18 p.
Keyword [en]
NOD mouse, Type 1 diabetes, IgVH gene complexity
National Category
Basic Medicine
Research subject
Immunology
Identifiers
URN: urn:nbn:se:umu:diva-87488DOI: 10.3109/08916939309004834PubMedID: 8105987OAI: oai:DiVA.org:umu-87488DiVA: diva2:709590
Available from: 2014-04-02 Created: 2014-04-02 Last updated: 2017-12-05Bibliographically approved
In thesis
1. Cellular and genetic components in the development of IDDM in NOD mice.
Open this publication in new window or tab >>Cellular and genetic components in the development of IDDM in NOD mice.
1996 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Umeå: Umeå universitet, 1996. 80 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 464
Keyword
non-obese diabetic, IgVh gene utilisation, immunoregulation, programmed cell death, genetic linkage analysis
National Category
Basic Medicine
Research subject
Immunology
Identifiers
urn:nbn:se:umu:diva-87787 (URN)91-7191-150-2 (ISBN)
Public defence
1996-03-22, Institutionen för Mikrobiologi, föreläsningssalen, Umeå universitet, Umeå, 09:30
Supervisors
Available from: 2014-04-11 Created: 2014-04-09 Last updated: 2014-04-11Bibliographically approved

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