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Specific destruction of islet transplants in NOD‹–›C57BL/6 and NOD‹–›C3H/Tif embryo aggregation chimeras irrespective of allelic differences in beta-cell antigens
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Department of Cell and Molecular Biology. University of Umeå)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Department of Cell and Molecular Biology, Umeå University, Umeå, Sweden)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Department of Cell and Molecular Biology, Umeå University, Umeå, Sweden)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Department of Cell and Molecular Biology. University of Umeå)
1995 (English)In: Journal of Autoimmunity, ISSN 0896-8411, E-ISSN 1095-9157, Vol. 8, no 3, 347-356 p.Article in journal (Refereed) Published
Abstract [en]

We have tested the hypothesis that allelic differences in the antigens expressed by the beta-cells of the islets of Langerhans influence the development of insulitis in the non-obese diabetic (NOD) mouse. Islets of Langerhans from NOD, C57BL/6 and C3H/Tif mice were transplanted under the kidney capsule of NOD<-->C57BL/6 and NOD<-->C3H/Tif embryo aggregation (EA) chimeras and the infiltration was scored 5-7 weeks later. Mononuclear cell infiltration of pancreatic islets was observed in 60% of the NOD<-->C57BL/6 and in 55% of the NOD<-->C3H/Tif EA chimeras. All transplanted EA chimeras that developed insulitis also displayed mononuclear cell infiltrates in the transplants, irrespective of the origin of the transplanted islets. In contrast, no infiltration of transplants was detected in EA chimeras scoring negative for insulitis. These results demonstrate that the specific destruction of islet transplants does not require the expression of NOD specific antigens by the islets. Moreover, the beta-cell destruction appears not to be restricted to NOD-MHC. The correlation between insulitis and transplant beta-cell destruction suggests the possibility that the development of insulitis is a prerequisite for transplant specific destruction. MHC restricted destruction may, therefore, precede the beta-cell destruction of transplanted islets. The chimerism among the mononuclear cells infiltrating the islet transplants was found to correlate with the overall haematopoetic chimerism in each of the individual EA chimeras. This observation suggests that NOD bone marrow, as well as non-NOD bone marrow, generates cells contributing to the beta-cell destruction process.

Place, publisher, year, edition, pages
Elsevier, 1995. Vol. 8, no 3, 347-356 p.
National Category
Basic Medicine Immunology
Research subject
Immunology
Identifiers
URN: urn:nbn:se:umu:diva-87503DOI: 10.1006/jaut.1995.0027PubMedID: 7575996OAI: oai:DiVA.org:umu-87503DiVA: diva2:709593
Available from: 2014-04-02 Created: 2014-04-02 Last updated: 2017-12-05Bibliographically approved
In thesis
1. Cellular and genetic components in the development of IDDM in NOD mice.
Open this publication in new window or tab >>Cellular and genetic components in the development of IDDM in NOD mice.
1996 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Umeå: Umeå universitet, 1996. 80 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 464
Keyword
non-obese diabetic, IgVh gene utilisation, immunoregulation, programmed cell death, genetic linkage analysis
National Category
Basic Medicine
Research subject
Immunology
Identifiers
urn:nbn:se:umu:diva-87787 (URN)91-7191-150-2 (ISBN)
Public defence
1996-03-22, Institutionen för Mikrobiologi, föreläsningssalen, Umeå universitet, Umeå, 09:30
Supervisors
Available from: 2014-04-11 Created: 2014-04-09 Last updated: 2014-04-11Bibliographically approved

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