Multispectral guided fluorescence diffuse optical tomography using upconverting nanoparticles
2014 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 104, no 7, 073703- p.Article in journal (Refereed) Published
We report on improved image detectability for fluorescence diffuse optical tomography using upconverting nanoparticles doped with rare-earth elements. Core-shell NaYF4 : Yb3+/Er3+ @ NaYF4 upconverting nanoparticles were synthesized through a stoichiometric method. The Yb3+/Er3+ sensitizer-activator pair yielded two anti-Stokes shifted fluorescence emission bands at 540 nm and 660 nm, here used to a priori estimate the fluorescence source depth with sub-millimeter precision. A spatially varying regularization incorporated the a priori fluorescence source depth estimation into the tomography reconstruction scheme. Tissue phantom experiments showed both an improved resolution and contrast in the reconstructed images as compared to not using any a priori information.
Place, publisher, year, edition, pages
2014. Vol. 104, no 7, 073703- p.
IdentifiersURN: urn:nbn:se:umu:diva-87649DOI: 10.1063/1.4863960ISI: 000332038500085OAI: oai:DiVA.org:umu-87649DiVA: diva2:710931