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Firing clamp: a novel method for single-trial estimation of excitatory and inhibitory synaptic neuronal conductances.
Computational Physics Laboratory, Division of Plasma Physics, Atomic Physics and Astrophysics, A.F. Ioffe Physical-Technical Institute of the Russian Academy of Sciences, St. Petersburg, Russia.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology. Department of Neurodynamics and Neurobiology, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia.
Neurophysiology and New Microscopies Laboratory, INSERM U603 - CNRS UMR 8154, Université Paris Descartes, Paris, France.
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2014 (English)In: Frontiers in cellular neuroscience, ISSN 1662-5102, Vol. 8, no 86, 86- p.Article in journal (Refereed) Published
Abstract [en]

Understanding non-stationary neuronal activity as seen in vivo requires estimation of both excitatory and inhibitory synaptic conductances from a single trial of recording. For this purpose, we propose a new intracellular recording method, called "firing clamp." Synaptic conductances are estimated from the characteristics of artificially evoked probe spikes, namely the spike amplitude and the mean subthreshold potential, which are sensitive to both excitatory and inhibitory synaptic input signals. The probe spikes, timed at a fixed rate, are evoked in the dynamic-clamp mode by injected meander-like current steps, with the step duration depending on neuronal membrane voltage. We test the method with perforated-patch recordings from isolated cells stimulated by external application or synaptic release of transmitter, and validate the method with simulations of a biophysically-detailed neuron model. The results are compared with the conductance estimates based on conventional current-clamp recordings.

Place, publisher, year, edition, pages
2014. Vol. 8, no 86, 86- p.
Keyword [en]
synaptic conductance estimation, dynamic clamp, firing-clamp
National Category
URN: urn:nbn:se:umu:diva-88025DOI: 10.3389/fncel.2014.00086ISI: 000333416900001PubMedID: 24734000OAI: diva2:713201
Available from: 2014-04-22 Created: 2014-04-22 Last updated: 2014-05-06Bibliographically approved

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Malinina, EvgeniaDruzin, MichaelJohansson, Staffan
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