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Gibberellins inhibit adventitious rooting in hybrid aspen and Arabidopsis by affecting auxin transport
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden ; Institut National de la Recherche Agronomique, Cestas Cedex, France.
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Institut Jean-Pierre Bourgin, UMR 1318 INRA–AgroParisTech, Institut National de la Recherche Agronomique Centre de Versailles–Grignon, Versailles Cedex, France.ORCID iD: 0000-0003-2985-6649
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
2014 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 78, no 3, 372-384 p.Article in journal (Refereed) Published
Abstract [en]

Knowledge of processes involved in adventitious rooting is important to improve both fundamental understanding of plant physiology and the propagation of numerous plants. Hybrid aspen (Populus tremula x tremuloides) plants overexpressing a key gibberellin (GA) biosynthesis gene (AtGA20ox1) grow rapidly but have poor rooting efficiency, which restricts their clonal propagation. Therefore, we investigated the molecular basis of adventitious rooting in Populus and the model plant Arabidopsis. The production of adventitious roots (ARs) in tree cuttings is initiated from the basal stem region, and involves the interplay of several endogenous and exogenous factors. The roles of several hormones in this process have been characterized, but the effects of GAs have not been fully investigated. Here, we show that a GA treatment negatively affects the numbers of ARs produced by wild-type hybrid aspen cuttings. Furthermore, both hybrid aspen plants and intact Arabidopsis seedlings overexpressing AtGA20ox1, PttGID1.1 or PttGID1.3 genes (with a 35S promoter) produce few ARs, although ARs develop from the basal stem region of hybrid aspen and the hypocotyl of Arabidopsis. In Arabidopsis, auxin and strigolactones are known to affect AR formation. Our data show that the inhibitory effect of GA treatment on adventitious rooting is not mediated by perturbation of the auxin signalling pathway, or of the strigolactone biosynthetic and signalling pathways. Instead, GAs appear to act by perturbing polar auxin transport, in particular auxin efflux in hybrid aspen, and both efflux and influx in Arabidopsis.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2014. Vol. 78, no 3, 372-384 p.
Keyword [en]
adventitious roots, gibberellins, Arabidopsis, Populus, jasmonate, auxin transport, auxin
National Category
Plant Biotechnology
Identifiers
URN: urn:nbn:se:umu:diva-89214DOI: 10.1111/tpj.12478ISI: 000334824300002OAI: oai:DiVA.org:umu-89214DiVA: diva2:719990
Available from: 2014-05-27 Created: 2014-05-26 Last updated: 2017-12-05Bibliographically approved

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