umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
DNA methylome profiling of human tissues identifies global and tissue-specific methylation patterns
Show others and affiliations
2014 (English)In: Genome Biology, ISSN 1465-6906, E-ISSN 1474-760X, Vol. 15, no 4, r54- p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: DNA epigenetic modifications, such as methylation, are important regulators of tissue differentiation, contributing to processes of both development and cancer. Profiling the tissue-specific DNA methylome patterns will provide novel insights into normal and pathogenic mechanisms, as well as help in future epigenetic therapies. In this study, 17 somatic tissues from four autopsied humans were subjected to functional genome analysis using the Illumina Infinium HumanMethylation450 BeadChip, covering 486 428 CpG sites. RESULTS: Only 2% of the CpGs analyzed are hypermethylated in all 17 tissue specimens; these permanently methylated CpG sites are located predominantly in gene-body regions. In contrast, 15% of the CpGs are hypomethylated in all specimens and are primarily located in regions proximal to transcription start sites. A vast number of tissue-specific differentially methylated regions are identified and considered likely mediators of tissue-specific gene regulatory mechanisms since the hypomethylated regions are closely related to known functions of the corresponding tissue. Finally, a clear inverse correlation is observed between promoter methylation within CpG islands and gene expression data obtained from publicly available databases. CONCLUSIONS: This genome-wide methylation profiling study identified tissue-specific differentially methylated regions in 17 human somatic tissues. Many of the genes corresponding to these differentially methylated regions contribute to tissue-specific functions. Future studies may use these data as a reference to identify markers of perturbed differentiation and disease-related pathogenic mechanisms.

Place, publisher, year, edition, pages
BioMed Central, 2014. Vol. 15, no 4, r54- p.
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Medical Genetics
Identifiers
URN: urn:nbn:se:umu:diva-91869DOI: 10.1186/gb-2014-15-4-r54ISI: 000338981500001OAI: oai:DiVA.org:umu-91869DiVA: diva2:738936
Available from: 2014-08-19 Created: 2014-08-18 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(1107 kB)114 downloads
File information
File name FULLTEXT01.pdfFile size 1107 kBChecksum SHA-512
f55870a6e5fc9cf176d7a404cede93cc868fe090bb2b197ee411d8571b67c70e6ac43f022e74ba55e5568c9c47f05e45a5490c19dccf839dac8b6c26023ef77c
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Nilsson, Torbjörn K

Search in DiVA

By author/editor
Nilsson, Torbjörn K
By organisation
Clinical chemistry
In the same journal
Genome Biology
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Medical Genetics

Search outside of DiVA

GoogleGoogle Scholar
Total: 114 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 93 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf