αβT Cell Receptors Expressed by CD4(-)CD8αβ(-) Intraepithelial T Cells Drive Their Fate into a Unique Lineage with Unusual MHC Reactivities
2014 (English)In: Immunity, ISSN 1074-7613, E-ISSN 1097-4180, Vol. 41, no 2, 207-218 p.Article in journal (Refereed) Published
Coreceptor CD4 and CD8αβ double-negative (DN) TCRαβ(+) intraepithelial T cells, although numerous, have been greatly overlooked and their contribution to the immune response is not known. Here we used T cell receptor (TCR) sequencing of single cells combined with retrogenic expression of TCRs to study the fate and the major histocompatibility complex (MHC) restriction of DN TCRαβ(+) intraepithelial T cells. The data show that commitment of thymic precursors to the DN TCRαβ(+) lineage is imprinted by their TCR specificity. Moreover, the TCRs they express display a diverse and unusual pattern of MHC restriction that is nonoverlapping with that of CD4(+) or CD8αβ(+) T cells, indicating that they sense antigens that are not recognized by the conventional T cell subsets. The new insights indicate that DN TCRαβ(+) T cells form a third lineage of TCRαβ T lymphocytes expressing a variable TCR repertoire, which serve nonredundant immune functions.
Place, publisher, year, edition, pages
2014. Vol. 41, no 2, 207-218 p.
Immunology in the medical area
IdentifiersURN: urn:nbn:se:umu:diva-92700DOI: 10.1016/j.immuni.2014.07.010ISI: 000341455300009PubMedID: 25131531OAI: oai:DiVA.org:umu-92700DiVA: diva2:742457