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Alterations in Gene Expression During Radiation-Induced Mitotic Catastrophe in He La Hep2 Cells
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Immunology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Immunology.
Umeå University, Faculty of Medicine, Department of Radiation Sciences, Radiation Physics.
Umeå University, Faculty of Medicine, Department of Radiation Sciences, Diagnostic Radiology.
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2014 (English)In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 34, no 8, 3875-3880 p.Article in journal (Refereed) Published
Abstract [en]

Aim: To explore kinetic changes in the gene expression profile during radiation-induced mitotic catastrophes. Materials and Methods: Gene expression changes were measured in HPV-infected HeLa Hep2 tumor cells following exposure to 5 Gy of ionizing radiation (Co-60). Signaling pathways were explored and correlated to the biological responses linked to mitotic catastrophe. Results: Following irradiation a transient G(2)-arrest was induced. Anaphase bridge formation and centrosome hyperamplification was observed. These phenotypical changes correlated well with the observed gene expression changes. Genes with altered expression were found to be involved in mitotic processes as well as G(2)- and spindle assembly checkpoints. Also centrosome-associated genes displayed an increased expression. Conclusion: This study elucidates specific characteristics in the altered gene expression pattern induced by irradiation, which can be correlated to the events of mitotic catastrophe in HeLa Hep2 cells. Therapeutic strategies modulating these alterations might potentiate future therapy and enhance tumor cell killing.

Place, publisher, year, edition, pages
INT INST ANTICANCER RESEARCH , 2014. Vol. 34, no 8, 3875-3880 p.
Keyword [en]
Radiation, cell cycle checkpoint, cell death, mitotic catastrophe, gene expression
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-92927ISI: 000339773400002OAI: oai:DiVA.org:umu-92927DiVA: diva2:748042
Available from: 2014-09-18 Created: 2014-09-09 Last updated: 2017-12-05Bibliographically approved

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Lindgren, TheresStigbrand, TorgnyJohansson, LennartRiklund, KatrineEriksson, David
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