umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
High-throughput sequencing shows inconsistent results with a microscope-based analysis of the soil prokaryotic community
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap. (Climate Impacts Research Centre (CIRC))
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för ekologi, miljö och geovetenskap. (Climate Impacts Research Centre (CIRC) ; Arcum)
Visa övriga samt affilieringar
2014 (Engelska)Ingår i: Soil Biology and Biochemistry, ISSN 0038-0717, E-ISSN 1879-3428, Vol. 76, s. 53-56Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

In the present study, we perform the first direct analysis on how the composition of the prokaryotic soil community differs depending on whether high-throughput sequencing or fluorescent in situ hybridization (FISH) coupled with catalyzed reporter deposition (CARD) is used. Soil samples were collected along short (<3 m) tundra vegetation gradients from Northern Sweden. Relative abundances of Acid-obacteria and Bacteroidetes estimated by the high-throughput sequencing were higher than those estimated by CARD-FISH, while relative abundances of Archaea and alpha-Proteobacteria estimated by high-throughput sequencing were lower than those estimated by CARD-FISH. The results indicated that the high-throughput sequencing overestimates/underestimates the relative abundance of some microbial taxa if we assume that CARD-FISH can provide potentially more quantitative data. Great caution should be taken when interpreting data generated by molecular technologies (both of high-throughput sequencing and CARD-FISH), and supports by multiple approaches are necessary to make a robust conclusion. (C) 2014 Elsevier Ltd. All rights reserved.

Ort, förlag, år, upplaga, sidor
Elsevier, 2014. Vol. 76, s. 53-56
Nyckelord [en]
Archaea, Bacteria, CARD-FISH, High-throughput sequencing, Soil prokaryotic community, Tundra ecosystem
Nationell ämneskategori
Ekologi Geovetenskap och miljövetenskap
Identifikatorer
URN: urn:nbn:se:umu:diva-92906DOI: 10.1016/j.soilbio.2014.05.010ISI: 000339695900007OAI: oai:DiVA.org:umu-92906DiVA, id: diva2:748189
Tillgänglig från: 2014-09-18 Skapad: 2014-09-09 Senast uppdaterad: 2018-06-07Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltext

Personposter BETA

Makoto, KobayashiKlaminder, Jonatan

Sök vidare i DiVA

Av författaren/redaktören
Makoto, KobayashiKlaminder, Jonatan
Av organisationen
Institutionen för ekologi, miljö och geovetenskap
I samma tidskrift
Soil Biology and Biochemistry
EkologiGeovetenskap och miljövetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 204 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf