Harnessing CRISPR-Cas9 immunity for genetic engineering
2014 (English)In: Current Opinion in Microbiology, ISSN 1369-5274, E-ISSN 1879-0364, Vol. 19, 114-119 p.Article in journal (Refereed) Published
CRISPR-Cas encodes an adaptive immune system that defends prokaryotes against infectious viruses and plasmids. Immunity is mediated by Cas nucleases, which use small RNA guides (the crRNAs) to specify a cleavage site within the genome of invading nucleic acids. In type II CRISPR-Cas systems, the DNA-cleaving activity is performed by a single enzyme Cas9 guided by an RNA duplex. Using synthetic single RNA guides, Cas9 can be reprogrammed to create specific double-stranded DNA breaks in the genomes of a variety of organisms, ranging from human cells to bacteria, and thus constitutes a powerful tool for genetic engineering. Here we describe recent advancements in our understanding of type II CRISPR-Cas immunity and how these studies led to revolutionary genome editing applications.
Place, publisher, year, edition, pages
2014. Vol. 19, 114-119 p.
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-93843DOI: 10.1016/j.mib.2014.07.001ISI: 000340984200018OAI: oai:DiVA.org:umu-93843DiVA: diva2:753226