Change search
ReferencesLink to record
Permanent link

Direct link
Genetic variation influences immune responses in sensitive rats following exposure to TiO2 nanoparticles
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Pulmonary Medicine. FOI.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Pulmonary Medicine.
Show others and affiliations
2014 (English)In: Toxicology, ISSN 0300-483X, E-ISSN 1879-3185, Vol. 326, 74-85 p.Article in journal (Other academic) Published
Abstract [en]

This study examines the immunological responses in rats following inhalation to titanium dioxide nanoparticles (TiO2 NPs), in naïve rats and in rats with induced allergic airway disease. The responses of two different inbred rat strains were compared: the Dark Aguoti (DA), susceptible to chronic inflammatory disorders, and the Brown Norwegian (BN), susceptible to atopic allergic inflammation. Naïve rats were exposed to an aerosol of TiO2 NPs once daily for 10 days. Another subset of rats was sensitized to the allergen ovalbumin (OVA) in order to induce airway inflammation. These sensitized rats were exposed to TiO2 NPs before and during the allergen challenge. Naïve rats exposed to TiO2 NPs developed an increase of neutrophils and lymphocytes in both rat strains. Airway hyperreactivity and production of inflammatory mediators typical of a T helper 1 type immune response were significantly increased, only in DA rats. Sensitization of the rats induced a prominent OVA-specific-IgE and IgG response in the BN rat while DA rats only showed an increased IgG response. Sensitized rats of both strains developed airway eosinophilia following allergen challenge, which declined upon exposure to TiO2 NPs. The level of neutrophils and lymphocytes increased upon exposure to TiO2 NPs in the airways of DA rats but remained unchanged in the airways of BN rats. In conclusion, the responses to TiO2 NPs were strain-dependent, indicating that genetics play a role in both immune and airway reactivity. DA rats were found to be higher responder compared to BN rats, both when it comes to responses in naïve and sensitized rats. The impact of genetically determined factors influencing the inflammatory reactions pinpoints the complexity of assessing health risks associated with nanoparticle exposures.

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 326, 74-85 p.
Keyword [en]
Titanium dioxide, Inflammation, Asthma, Ovalbumin, Aerosol inhalation, Immune response
National Category
Respiratory Medicine and Allergy Pharmacology and Toxicology
Research subject
Toxicology; Occupational and Environmental Medicine; Lung Medicine; nanoparticles; nanotoxicology; Immunology
URN: urn:nbn:se:umu:diva-95723DOI: 10.1016/j.tox.2014.10.004ISI: 000347133400009OAI: diva2:760677
Swedish Research Council FormasForte, Swedish Research Council for Health, Working Life and Welfare

Forskningsfinansiär: Umeå Center for Environmental Research, and by the Swedish Ministry of Defence

Available from: 2014-11-04 Created: 2014-11-04 Last updated: 2015-03-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Gustafsson, ÅsaSandström, ThomasBucht, Anders
By organisation
Pulmonary Medicine
In the same journal
Respiratory Medicine and AllergyPharmacology and Toxicology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 55 hits
ReferencesLink to record
Permanent link

Direct link